Summary of Study ST002237

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001427. The data can be accessed directly via it's Project DOI: 10.21228/M88D9X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002237
Study TitleMetabolomic analysis of brain cortex from neuronal specific Depdc5 knockout in fed and fasting state
Study TypeDepdc5 KO fed vs. fasted comparison
Study SummaryCaloric restriction and acute fasting are known to reduce seizures but through unclear mechanisms. In this study, we demonstrate that mTORC1 signaling is reduced after acute fasting of mice. In neurons, mTORC1 is most sensitive to withdrawal of leucine, arginine, and glutamine, which is dependent on DEPDC5. We performed metabolomic analysis of brain cortex from neuronal specific Depdc5 knockout in fed and fasting state. The Depdc5 neuronal specific knockout mice are resistant to sensing significant fluctuations in brain amino acid levels after fasting. These results establish that acute fasting reduces seizure susceptibility in a DEPDC5-dependent manner.
Institute
Northwestern University, Feinberg School of Medicine
DepartmentMedicine
LaboratoryChandel Lab
Last NameChandel
First NameNavdeep
Address303 E Superior St, Chicago, Illinois, 60611, USA
Emailnav@northwestern.edu
Phone3125032549
Submit Date2022-07-27
Num Groups4
Total Subjects40
Num Males20
Num Females20
PublicationsDEPDC5-dependent mTORC1 signaling mechanisms are critical for the anti-seizure effects of acute fasting
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-08-15
Release Version1
Navdeep Chandel Navdeep Chandel
https://dx.doi.org/10.21228/M88D9X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Treatment
SA213107600-1Depdc5 KO Control
SA213108609-6Depdc5 KO Control
SA213109248-7Depdc5 KO Control
SA213110192-2Depdc5 KO Control
SA213111192-4Depdc5 KO Control
SA213112192-5Depdc5 KO Control
SA213113189-2Depdc5 KO Control
SA213114167-7Depdc5 KO Control
SA213115993-10Depdc5 KO Control
SA213116625-1Depdc5 KO Control
SA213117986-5Depdc5 KO Fasted
SA213118192-6Depdc5 KO Fasted
SA213119986-3Depdc5 KO Fasted
SA213120611-5Depdc5 KO Fasted
SA213121627-7Depdc5 KO Fasted
SA213122601-5Depdc5 KO Fasted
SA213123248-6Depdc5 KO Fasted
SA213124166-6Depdc5 KO Fasted
SA213125192-1Depdc5 KO Fasted
SA213126169-3Depdc5 KO Fasted
SA213127993-1Depdc5 WT Control
SA213128189-3Depdc5 WT Control
SA213129169-2Depdc5 WT Control
SA213130169-5Depdc5 WT Control
SA213131601-1Depdc5 WT Control
SA213132166-3Depdc5 WT Control
SA213133166-4Depdc5 WT Control
SA213134191-3Depdc5 WT Control
SA213135191-1Depdc5 WT Control
SA213136993-4Depdc5 WT Control
SA213137993-5Depdc5 WT Fasted
SA213138166-5Depdc5 WT Fasted
SA213139190-4Depdc5 WT Fasted
SA213140600-7Depdc5 WT Fasted
SA213141248-1Depdc5 WT Fasted
SA213142600-8Depdc5 WT Fasted
SA213143601-2Depdc5 WT Fasted
SA213144190-5Depdc5 WT Fasted
SA213145601-4Depdc5 WT Fasted
SA213146627-6Depdc5 WT Fasted
Showing results 1 to 40 of 40
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