Summary of Study ST002283

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001463. The data can be accessed directly via it's Project DOI: 10.21228/M8MH6X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002283
Study TitleThe “ForensOMICS” approach to forensic post-mortem interval estimation: combining metabolomics, lipidomics and proteomics for the analysis human skeletal remains
Study SummaryThe combined use of multiple omics methods to answer complex system biology questions is growing in biological and medical sciences, as the importance of studying interrelated biological processes in their entirety is increasingly recognized. We applied a combination of metabolomics, lipidomics and proteomics to human bone to investigate the potential of this multi-omics approach to estimate the time elapsed since death (i.e., the post-mortem interval, PMI). This “ForensOMICS” approach has the potential to improve accuracy and precision of PMI estimation of skeletonized human remains, thereby helping forensic investigators to establish the timeline of events surrounding death. Anterior midshaft tibial bone was collected from four female body donors in a fresh stage of decomposition before placement of the bodies to decompose outdoors at the human taphonomy facility managed by the Forensic Anthropological Center at Texas State (FACTS). Bone samples were again collected at selected PMIs (219, 790, 834 and 872 days). Liquid chromatography mass spectrometry (LC-MS) was used to obtain untargeted metabolomic, lipidomic and proteomic profiles from the pre- and post-placement bone samples. Multivariate analysis was used to investigate the three omics blocks by means of Data Integration Analysis for Biomarker discovery using Latent variable approaches for Omics studies (DIABLO), to identify the reduced number of markers that could effectively describe post-mortem changes and classify the individuals based on their PMI. The resulting model showed that pre-placement bone metabolome, lipidome and proteome profiles were clearly distinguishable from post-placement profiles. Metabolites associated with the pre-placement samples, suggested an extinction of the energetic metabolism and a switch towards another source of fuelling (e.g., structural proteins). We were able to identify certain biomolecules from the three groups that show excellent potential for estimation of the PMI, predominantly the biomolecules from the metabolomics block. Our findings suggest that, by targeting a combination of compounds with different post-mortem stability, in future studies we could be able to estimate both short PMIs, by using metabolites and lipids, and longer PMIs, by including more stable proteins.
Institute
University of Central Lancashire
Last NameBonicelli
First NameAndrea
AddressFylde Rd, Preston PR1 2HE
Emailabonicelli@uclan.ac.uk
Phone07383974949
Submit Date2022-09-06
Num Groups5
Total Subjects4
Num Females4
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-10-13
Release Version1
Andrea Bonicelli Andrea Bonicelli
https://dx.doi.org/10.21228/M8MH6X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Age PMI Deposition State
SA218507D3_TF_D_Lip_Neg61 2-10 Pre deposition Fresh
SA218508D3_TF_C_Lip_Neg61 2-10 Pre deposition Fresh
SA218509D3_TF_A_Hilic_Neg61 2-10 Pre deposition Fresh
SA218510D3_TF_E_Lip_Neg61 2-10 Pre deposition Fresh
SA218511D3_TF_D_Hilic_Pos61 2-10 Pre deposition Fresh
SA218512D3_TF_B_Hilic_Pos61 2-10 Pre deposition Fresh
SA218513D3_TF_C_Hilic_Pos61 2-10 Pre deposition Fresh
SA218514D3_TF_C_Hilic_Neg61 2-10 Pre deposition Fresh
SA218515D3_TF_E_Hilic_Pos61 2-10 Pre deposition Fresh
SA218516D3_TF_E_Hilic_Neg61 2-10 Pre deposition Fresh
SA218517D3_TF_C_Lip_Pos61 2-10 Pre deposition Fresh
SA218518D3_TF_D_Lip_Pos61 2-10 Pre deposition Fresh
SA218519D3_TF_E_Lip_Pos61 2-10 Pre deposition Fresh
SA218520D3_TF_B_Lip_Pos61 2-10 Pre deposition Fresh
SA218521D3_TF_A_Lip_Pos61 2-10 Pre deposition Fresh
SA218522D3_TF_A_Hilic_Pos61 2-10 Pre deposition Fresh
SA218523D3_TF_B_Lip_Neg61 2-10 Pre deposition Fresh
SA218524D3_TF_A_Lip_Neg61 2-10 Pre deposition Fresh
SA218525D3_TF_D_Hilic_Neg61 2-10 Pre deposition Fresh
SA218526D3_TF_B_Hilic_Neg61 2-10 Pre deposition Fresh
SA218527D3_TS_A_Lip_Pos61 834 Burial Skeletonised
SA218528D3_TS_E_Lip_Neg61 834 Burial Skeletonised
SA218529D3_TS_B_Lip_Pos61 834 Burial Skeletonised
SA218530D3_TS_C_Lip_Pos61 834 Burial Skeletonised
SA218531D3_TS_E_Lip_Pos61 834 Burial Skeletonised
SA218532D3_TS_D_Lip_Pos61 834 Burial Skeletonised
SA218533D3_TS_D_Lip_Neg61 834 Burial Skeletonised
SA218534D3_TS_C_Lip_Neg61 834 Burial Skeletonised
SA218535D3_TS_B_Hilic_Neg61 834 Burial Skeletonised
SA218536D3_TS_A_Hilic_Neg61 834 Burial Skeletonised
SA218537D3_TS_C_Hilic_Neg61 834 Burial Skeletonised
SA218538D3_TS_A_Lip_Neg61 834 Burial Skeletonised
SA218539D3_TS_B_Lip_Neg61 834 Burial Skeletonised
SA218540D3_TS_E_Hilic_Neg61 834 Burial Skeletonised
SA218541D3_TS_D_Hilic_Neg61 834 Burial Skeletonised
SA218542D3_TS_D_Hilic_Pos61 834 Burial Skeletonised
SA218543D3_TS_B_Hilic_Pos61 834 Burial Skeletonised
SA218544D3_TS_A_Hilic_Pos61 834 Burial Skeletonised
SA218545D3_TS_E_Hilic_Pos61 834 Burial Skeletonised
SA218546D3_TS_C_Hilic_Pos61 834 Burial Skeletonised
SA218547D2_TF_E_Lip_Neg67 2-10 Pre deposition Fresh
SA218548D2_TF_D_Lip_Neg67 2-10 Pre deposition Fresh
SA218549D2_TF_A_Hilic_Pos67 2-10 Pre deposition Fresh
SA218550D2_TF_B_Hilic_Pos67 2-10 Pre deposition Fresh
SA218551D2_TF_C_Hilic_Pos67 2-10 Pre deposition Fresh
SA218552D2_TF_C_Lip_Neg67 2-10 Pre deposition Fresh
SA218553D2_TF_E_Lip_Pos67 2-10 Pre deposition Fresh
SA218554D2_TF_B_Lip_Pos67 2-10 Pre deposition Fresh
SA218555D2_TF_C_Lip_Pos67 2-10 Pre deposition Fresh
SA218556D2_TF_E_Hilic_Neg67 2-10 Pre deposition Fresh
SA218557D2_TF_D_Lip_Pos67 2-10 Pre deposition Fresh
SA218558D2_TF_A_Lip_Pos67 2-10 Pre deposition Fresh
SA218559D2_TF_D_Hilic_Neg67 2-10 Pre deposition Fresh
SA218560D2_TF_E_Hilic_Pos67 2-10 Pre deposition Fresh
SA218561D2_TF_B_Hilic_Neg67 2-10 Pre deposition Fresh
SA218562D2_TF_A_Hilic_Neg67 2-10 Pre deposition Fresh
SA218563D2_TF_C_Hilic_Neg67 2-10 Pre deposition Fresh
SA218564D2_TF_B_Lip_Neg67 2-10 Pre deposition Fresh
SA218565D2_TF_D_Hilic_Pos67 2-10 Pre deposition Fresh
SA218566D2_TF_A_Lip_Neg67 2-10 Pre deposition Fresh
SA218567D2_TS_E_Hilic_Pos67 790 Burial Skeletonised
SA218568D2_TS_B_Lip_Pos67 790 Burial Skeletonised
SA218569D2_TS_A_Lip_Pos67 790 Burial Skeletonised
SA218570D2_TS_D_Lip_Neg67 790 Burial Skeletonised
SA218571D2_TS_C_Hilic_Pos67 790 Burial Skeletonised
SA218572D2_TS_B_Hilic_Pos67 790 Burial Skeletonised
SA218573D2_TS_E_Lip_Neg67 790 Burial Skeletonised
SA218574D2_TS_C_Lip_Pos67 790 Burial Skeletonised
SA218575D2_TS_C_Lip_Neg67 790 Burial Skeletonised
SA218576D2_TS_A_Hilic_Pos67 790 Burial Skeletonised
SA218577D2_TS_D_Hilic_Neg67 790 Burial Skeletonised
SA218578D2_TS_C_Hilic_Neg67 790 Burial Skeletonised
SA218579D2_TS_B_Hilic_Neg67 790 Burial Skeletonised
SA218580D2_TS_A_Hilic_Neg67 790 Burial Skeletonised
SA218581D2_TS_E_Hilic_Neg67 790 Burial Skeletonised
SA218582D2_TS_E_Lip_Pos67 790 Burial Skeletonised
SA218583D2_TS_B_Lip_Neg67 790 Burial Skeletonised
SA218584D2_TS_D_Hilic_Pos67 790 Burial Skeletonised
SA218585D2_TS_D_Lip_Pos67 790 Burial Skeletonised
SA218586D2_TS_A_Lip_Neg67 790 Burial Skeletonised
SA218587D4_TF_B_Lip_Pos77 2-10 Pre deposition Fresh
SA218588D4_TF_A_Lip_Pos77 2-10 Pre deposition Fresh
SA218589D4_TF_C_Lip_Pos77 2-10 Pre deposition Fresh
SA218590D4_TF_D_Lip_Pos77 2-10 Pre deposition Fresh
SA218591D4_TF_E_Lip_Pos77 2-10 Pre deposition Fresh
SA218592D4_TF_B_Hilic_Neg77 2-10 Pre deposition Fresh
SA218593D4_TF_D_Hilic_Pos77 2-10 Pre deposition Fresh
SA218594D4_TF_C_Hilic_Pos77 2-10 Pre deposition Fresh
SA218595D4_TF_C_Lip_Neg77 2-10 Pre deposition Fresh
SA218596D4_TF_E_Hilic_Pos77 2-10 Pre deposition Fresh
SA218597D4_TF_E_Lip_Neg77 2-10 Pre deposition Fresh
SA218598D4_TF_B_Lip_Neg77 2-10 Pre deposition Fresh
SA218599D4_TF_A_Lip_Neg77 2-10 Pre deposition Fresh
SA218600D4_TF_C_Hilic_Neg77 2-10 Pre deposition Fresh
SA218601D4_TF_A_Hilic_Neg77 2-10 Pre deposition Fresh
SA218602D4_TF_D_Lip_Neg77 2-10 Pre deposition Fresh
SA218603D4_TF_B_Hilic_Pos77 2-10 Pre deposition Fresh
SA218604D4_TF_E_Hilic_Neg77 2-10 Pre deposition Fresh
SA218605D4_TF_D_Hilic_Neg77 2-10 Pre deposition Fresh
SA218606D4_TF_A_Hilic_Pos77 2-10 Pre deposition Fresh
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