Summary of Study ST002352

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002352
Study TitleBiomolecular condensates create phospholipid-enriched microenvironments (Part 2)
Study TypeMetabolomes of in vitro synthesized condensates
Study SummaryProteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1.
Institute
Cornell University
DepartmentDepartment of Pharmacology
LaboratoryDr. Samie Jaffrey
Last NameDumelie
First NameJason
Address1300 York Ave, LC-524, New York City, NY
Emailjdumes98@gmail.com
Phone6465690174
Submit Date2022-11-15
Raw Data AvailableYes
Raw Data File Type(s)mzdata.xml
Analysis Type DetailLC-MS
Release Date2023-03-01
Release Version2
Jason Dumelie Jason Dumelie
https://dx.doi.org/10.21228/M8N71K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Protein RNA fraction Extraction from condensate timing of metabolite extract addition
SA249743MED1 Aqueous Sample 6MED1 150 nM aqueous No heat step standard
SA249744MED1 Aqueous Sample 5MED1 150 nM aqueous No heat step standard
SA249745MED1 Aqueous Sample 4MED1 150 nM aqueous No heat step standard
SA249746MED1 Aqueous Sample 8MED1 150 nM aqueous standard Metabolites added after 10 min incubation
SA249747MED1 Aqueous Sample 9MED1 150 nM aqueous standard Metabolites added after 10 min incubation
SA249748MED1 Aqueous Sample 7MED1 150 nM aqueous standard Metabolites added after 10 min incubation
SA249749MED1 Condensate Sample 5MED1 150 nM condensate No heat step standard
SA249750MED1 Condensate Sample 6MED1 150 nM condensate No heat step standard
SA249751MED1 Condensate Sample 4MED1 150 nM condensate No heat step standard
SA249752MED1 Condensate Sample 8MED1 150 nM condensate standard Metabolites added after 10 min incubation
SA249753MED1 Condensate Sample 9MED1 150 nM condensate standard Metabolites added after 10 min incubation
SA249754MED1 Condensate Sample 7MED1 150 nM condensate standard Metabolites added after 10 min incubation
SA249755MED1 Input Sample 5MED1 150 nM input No heat step standard
SA249756MED1 Input Sample 4MED1 150 nM input No heat step standard
SA249757MED1 Input Sample 6MED1 150 nM input No heat step standard
SA249758MED1 Input Sample 7MED1 150 nM input standard Metabolites added after 10 min incubation
SA249759MED1 Input Sample 8MED1 150 nM input standard Metabolites added after 10 min incubation
SA249760MED1 Input Sample 9MED1 150 nM input standard Metabolites added after 10 min incubation
Showing results 1 to 18 of 18
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