Summary of Study ST003050

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001899. The data can be accessed directly via it's Project DOI: 10.21228/M88147 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003050
Study TitlePlasma instead of serum avoids critical confounding of clinical metabolomics studies by platelets (Part 1/3 - Plasma and serum eicosadomics)
Study SummaryMetabolomics is an emerging and powerful molecular profiling method supporting clinical investigations. Serum and plasma are commonly used without rational prioritization. Serum is collected after blood coagulation, a complex biochemical process involving active platelet metabolism. This may affect the metabolome and increase the variance as platelet counts and function may vary substantially in individuals. A multi-omics approach systematically investigating the suitability of serum and plasma for clinical studies demonstrated that metabolites correlated well (n=461, R2=0.991), whereas lipid mediators (n=104, R2=0.906) and proteins (n=322, R2=0.860) differed substantially between specimen. Independently, analysis of platelet releasates identified most biomolecules significantly enriched in serum when compared to plasma. A prospective, randomized, controlled parallel group metabolomics trial with acetylsalicylic acid administered for 7 days demonstrated that the apparent drug effects significantly differ depending on analyzed specimen. Only serum analyses of healthy individuals suggested a significant downregulation of TXB2 and 12-HETE, which were specifically formed during coagulation in vitro. Plasma analyses reliably identified acetylsalicylic acid effects on metabolites and lipids occurring in vivo such as a decrease in polyunsaturated fatty acids. The present data suggests that plasma should be preferred above serum for clinical metabolomics studies as the serum metabolome may be substantially confounded by platelets.
Institute
University of Vienna
DepartmentDepartment of Analytical Chemistry
LaboratoryGerner lab
Last NameHagn
First NameGerhard
AddressWähringerstraße 38, 1090 Vienna, Austria
Emailgerhard.hagn@univie.ac.at
Phone+43 1 4277 52375
Submit Date2024-01-17
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-04-12
Release Version1
Gerhard Hagn Gerhard Hagn
https://dx.doi.org/10.21228/M88147
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample material Treatment
SA331296Donor_1_Plasma_after_Aspirin_1Plasma after_Aspirin
SA331297Donor_5_Plasma_after_Aspirin_1Plasma after_Aspirin
SA331298Donor_6_Plasma_after_Aspirin_1Plasma after_Aspirin
SA331299Donor_6_Plasma_after_Aspirin_2Plasma after_Aspirin
SA331300Donor_1_Plasma_after_Aspirin_2Plasma after_Aspirin
SA331301Donor_4_Plasma_after_Aspirin_2Plasma after_Aspirin
SA331302Donor_5_Plasma_after_Aspirin_2Plasma after_Aspirin
SA331303Donor_2_Plasma_after_Aspirin_1Plasma after_Aspirin
SA331304Donor_4_Plasma_after_Aspirin_1Plasma after_Aspirin
SA331305Donor_2_Plasma_after_Aspirin_2Plasma after_Aspirin
SA331306Donor_3_Plasma_after_Aspirin_2Plasma after_Aspirin
SA331307Donor_3_Plasma_after_Aspirin_1Plasma after_Aspirin
SA331308Donor_9_Plasma_after_Omega3_2Plasma after_Omega3
SA331309Donor_9_Plasma_after_Omega3_1Plasma after_Omega3
SA331310Donor_7_Plasma_after_Omega3_1Plasma after_Omega3
SA331311Donor_8_Plasma_after_Omega3_1Plasma after_Omega3
SA331312Donor_8_Plasma_after_Omega3_2Plasma after_Omega3
SA331313Donor_12_Plasma_after_Omega3_2Plasma after_Omega3
SA331314Donor_12_Plasma_after_Omega3_1Plasma after_Omega3
SA331315Donor_7_Plasma_after_Omega3_2Plasma after_Omega3
SA331316Donor_11_Plasma_after_Omega3_2Plasma after_Omega3
SA331317Donor_11_Plasma_after_Omega3_1Plasma after_Omega3
SA331318Donor_10_Plasma_after_Omega3_2Plasma after_Omega3
SA331319Donor_10_Plasma_after_Omega3_1Plasma after_Omega3
SA331320Donor_4_Plasma_before_Aspirin_1Plasma before_Aspirin
SA331321Donor_2_Plasma_before_Aspirin_2Plasma before_Aspirin
SA331322Donor_2_Plasma_before_Aspirin_1Plasma before_Aspirin
SA331323Donor_1_Plasma_before_Aspirin_1Plasma before_Aspirin
SA331324Donor_1_Plasma_before_Aspirin_2Plasma before_Aspirin
SA331325Donor_3_Plasma_before_Aspirin_1Plasma before_Aspirin
SA331326Donor_3_Plasma_before_Aspirin_2Plasma before_Aspirin
SA331327Donor_6_Plasma_before_Aspirin_1Plasma before_Aspirin
SA331328Donor_5_Plasma_before_Aspirin_2Plasma before_Aspirin
SA331329Donor_5_Plasma_before_Aspirin_1Plasma before_Aspirin
SA331330Donor_6_Plasma_before_Aspirin_2Plasma before_Aspirin
SA331331Donor_4_Plasma_before_Aspirin_2Plasma before_Aspirin
SA331332Donor_8_Plasma_before_Omega3_2Plasma before_Omega3
SA331333Donor_9_Plasma_before_Omega3_2Plasma before_Omega3
SA331334Donor_8_Plasma_before_Omega3_1Plasma before_Omega3
SA331335Donor_7_Plasma_before_Omega3_2Plasma before_Omega3
SA331336Donor_7_Plasma_before_Omega3_1Plasma before_Omega3
SA331337Donor_10_Plasma_before_Omega3_1Plasma before_Omega3
SA331338Donor_9_Plasma_before_Omega3_1Plasma before_Omega3
SA331339Donor_12_Plasma_before_Omega3_1Plasma before_Omega3
SA331340Donor_10_Plasma_before_Omega3_2Plasma before_Omega3
SA331341Donor_11_Plasma_before_Omega3_2Plasma before_Omega3
SA331342Donor_12_Plasma_before_Omega3_2Plasma before_Omega3
SA331343Donor_11_Plasma_before_Omega3_1Plasma before_Omega3
SA331284Donor_3_Plasma_2Plasma Plasma_Serum_comparison
SA331285Donor_3_Plasma_1Plasma Plasma_Serum_comparison
SA331286Donor_2_Plasma_1Plasma Plasma_Serum_comparison
SA331287Donor_4_Plasma_1Plasma Plasma_Serum_comparison
SA331288Donor_2_Plasma_2Plasma Plasma_Serum_comparison
SA331289Donor_5_Plasma_1Plasma Plasma_Serum_comparison
SA331290Donor_6_Plasma_2Plasma Plasma_Serum_comparison
SA331291Donor_6_Plasma_1Plasma Plasma_Serum_comparison
SA331292Donor_5_Plasma_2Plasma Plasma_Serum_comparison
SA331293Donor_1_Plasma_2Plasma Plasma_Serum_comparison
SA331294Donor_4_Plasma_2Plasma Plasma_Serum_comparison
SA331295Donor_1_Plasma_1Plasma Plasma_Serum_comparison
SA331356Donor_5_Serum_after_Aspirin_1Serum after_Aspirin
SA331357Donor_5_Serum_after_Aspirin_2Serum after_Aspirin
SA331358Donor_4_Serum_after_Aspirin_2Serum after_Aspirin
SA331359Donor_6_Serum_after_Aspirin_2Serum after_Aspirin
SA331360Donor_6_Serum_after_Aspirin_1Serum after_Aspirin
SA331361Donor_1_Serum_after_Aspirin_1Serum after_Aspirin
SA331362Donor_2_Serum_after_Aspirin_1Serum after_Aspirin
SA331363Donor_4_Serum_after_Aspirin_1Serum after_Aspirin
SA331364Donor_2_Serum_after_Aspirin_2Serum after_Aspirin
SA331365Donor_1_Serum_after_Aspirin_2Serum after_Aspirin
SA331366Donor_3_Serum_after_Aspirin_2Serum after_Aspirin
SA331367Donor_3_Serum_after_Aspirin_1Serum after_Aspirin
SA331368Donor_9_Serum_after_Omega3_1Serum after_Omega3
SA331369Donor_9_Serum_after_Omega3_2Serum after_Omega3
SA331370Donor_8_Serum_after_Omega3_2Serum after_Omega3
SA331371Donor_8_Serum_after_Omega3_1Serum after_Omega3
SA331372Donor_10_Serum_after_Omega3_1Serum after_Omega3
SA331373Donor_7_Serum_after_Omega3_1Serum after_Omega3
SA331374Donor_12_Serum_after_Omega3_1Serum after_Omega3
SA331375Donor_7_Serum_after_Omega3_2Serum after_Omega3
SA331376Donor_12_Serum_after_Omega3_2Serum after_Omega3
SA331377Donor_11_Serum_after_Omega3_2Serum after_Omega3
SA331378Donor_11_Serum_after_Omega3_1Serum after_Omega3
SA331379Donor_10_Serum_after_Omega3_2Serum after_Omega3
SA331380Donor_1_Serum_before_Aspirin_1Serum before_Aspirin
SA331381Donor_4_Serum_before_Aspirin_2Serum before_Aspirin
SA331382Donor_5_Serum_before_Aspirin_1Serum before_Aspirin
SA331383Donor_5_Serum_before_Aspirin_2Serum before_Aspirin
SA331384Donor_6_Serum_before_Aspirin_1Serum before_Aspirin
SA331385Donor_4_Serum_before_Aspirin_1Serum before_Aspirin
SA331386Donor_3_Serum_before_Aspirin_2Serum before_Aspirin
SA331387Donor_1_Serum_before_Aspirin_2Serum before_Aspirin
SA331388Donor_2_Serum_before_Aspirin_1Serum before_Aspirin
SA331389Donor_2_Serum_before_Aspirin_2Serum before_Aspirin
SA331390Donor_3_Serum_before_Aspirin_1Serum before_Aspirin
SA331391Donor_6_Serum_before_Aspirin_2Serum before_Aspirin
SA331392Donor_7_Serum_before_Omega3_1Serum before_Omega3
SA331393Donor_10_Serum_before_Omega3_2Serum before_Omega3
SA331394Donor_11_Serum_before_Omega3_1Serum before_Omega3
SA331395Donor_11_Serum_before_Omega3_2Serum before_Omega3
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