Summary of Study ST003137

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001950. The data can be accessed directly via it's Project DOI: 10.21228/M8NX50 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003137
Study Title	Lipid class-specific kinetics of plasma fatty acids, oxylipins, endocannabinoids, lysophospholipids and bile acids upon a lipopolysaccharide challenge of healthy humans and their modulation by anti-oxidative supplements (Part 1/2 - negative mode)
Study Summary	While molecular mechanisms of inflammatory processes are well characterized, the systemic responses of humans exposed to pathogen-associated molecular pattern with regard to fatty acid derivatives and other lipids have hardly been determined. Here, we present a dual stage controlled clinical intervention study with healthy individuals challenged with lipopolysaccharide. While in a first stage, plasma proteomics and lipidomics was applied to observe the kinetics of inflammatory modulators within eight hours, the effects of a placebocontrolled anti-oxidative intervention were determined in the second stage. Plasma proteome profiling demonstrated the early involvement of platelets detectable within two hours after lipopolysaccharide challenge, followed by the characteristic induction of liver-derived acute phase proteins and innate immune cell-derived alarmins. Untargeted lipidomics demonstrated the early release of fatty acids and taurocholic acid within two hours, followed by complex time courses of various oxylipins and the downregulation of numerous lysophospholipids and deoxycholic acid. Groups of molecules with similar kinetics during the time course analysis upon lipopolysaccharide challenge were observed to have common precursors or synthesizing enzymes. Dietary supplementation with antioxidants did not affect the kinetics of detectable proteins, but significantly downregulated the pro-inflammatory sphingosine-1-phosphate and increased the levels of oxylipins described to facilitate the resolution of inflammation, 20-HEPE and 22-HDoHE. The present study identified a complex network of oxylipins, bile acids, lysophospholipids and endocannabinoids deregulated in plasma upon lipopolysaccharide challenge, introduces platelets as powerful inflammatory modulators and suggests that dietary antioxidant supplementation hardly interferes with the induction of inflammatory processes, but may rather support the resolution of inflammation.
Institute	University of Vienna
Department	Department of Analytical Chemistry
Laboratory	Gerner lab
Last Name	Hagn
First Name	Gerhard
Address	Währingerstraße 38, 1090 Vienna, Austria
Email	gerhard.hagn@univie.ac.at
Phone	+43 1 4277 52375
Submit Date	2024-03-18
Raw Data Available	Yes
Raw Data File Type(s)	mzML, raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2025-06-02
Release Version	1

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Treatment
SA339888	Donor33_stage1_480_min_con_1	con
SA339889	Donor25_stage1_480_min_con_2	con
SA339890	Donor25_stage1_480_min_con_1	con
SA339891	Donor25_stage1_240_min_con_2	con
SA339892	Donor25_stage1_240_min_con_1	con
SA339893	Donor33_stage1_120_min_con_2	con
SA339894	Donor33_stage1_240_min_con_1	con
SA339895	Donor25_stage1_120_min_con_2	con
SA339896	Donor24_stage1_60_min_con_1	con
SA339897	Donor33_stage1_0_min_con_1	con
SA339898	Donor33_stage1_480_min_con_2	con
SA339899	Donor33_stage1_240_min_con_2	con
SA339900	Donor33_stage1_0_min_con_2	con
SA339901	Donor32_stage1_60_min_con_1	con
SA339902	Donor24_stage1_240_min_con_1	con
SA339903	Donor24_stage1_120_min_con_2	con
SA339904	Donor24_stage1_120_min_con_1	con
SA339905	Donor24_stage1_60_min_con_2	con
SA339906	Donor24_stage1_240_min_con_2	con
SA339907	Donor24_stage1_480_min_con_1	con
SA339908	Donor24_stage1_480_min_con_2	con
SA339909	Donor25_stage1_0_min_con_1	con
SA339910	Donor25_stage1_0_min_con_2	con
SA339911	Donor25_stage1_60_min_con_1	con
SA339912	Donor32_stage1_480_min_con_2	con
SA339913	Donor33_stage1_60_min_con_1	con
SA339914	Donor32_stage1_60_min_con_2	con
SA339915	Donor33_stage1_60_min_con_2	con
SA339916	Donor33_stage1_120_min_con_1	con
SA339917	Donor25_stage1_60_min_con_2	con
SA339918	Donor32_stage1_120_min_con_1	con
SA339919	Donor32_stage1_120_min_con_2	con
SA339920	Donor32_stage1_480_min_con_1	con
SA339921	Donor32_stage1_240_min_con_2	con
SA339922	Donor32_stage1_240_min_con_1	con
SA339923	Donor25_stage1_120_min_con_1	con
SA339924	Donor26_stage1_480_min_con_1	con
SA339925	Donor28_stage1_0_min_con_1	con
SA339926	Donor27_stage1_480_min_con_2	con
SA339927	Donor28_stage1_0_min_con_2	con
SA339928	Donor29_stage1_480_min_con_2	con
SA339929	Donor29_stage1_240_min_con_2	con
SA339930	Donor29_stage1_480_min_con_1	con
SA339931	Donor27_stage1_480_min_con_1	con
SA339932	Donor27_stage1_240_min_con_2	con
SA339933	Donor30_stage1_0_min_con_1	con
SA339934	Donor30_stage1_0_min_con_2	con
SA339935	Donor27_stage1_60_min_con_2	con
SA339936	Donor27_stage1_120_min_con_1	con
SA339937	Donor27_stage1_240_min_con_1	con
SA339938	Donor27_stage1_120_min_con_2	con
SA339939	Donor29_stage1_240_min_con_1	con
SA339940	Donor29_stage1_120_min_con_2	con
SA339941	Donor28_stage1_480_min_con_1	con
SA339942	Donor28_stage1_240_min_con_2	con
SA339943	Donor28_stage1_480_min_con_2	con
SA339944	Donor24_stage1_0_min_con_2	con
SA339945	Donor29_stage1_0_min_con_1	con
SA339946	Donor29_stage1_0_min_con_2	con
SA339947	Donor28_stage1_240_min_con_1	con
SA339948	Donor28_stage1_120_min_con_2	con
SA339949	Donor29_stage1_60_min_con_2	con
SA339950	Donor29_stage1_120_min_con_1	con
SA339951	Donor29_stage1_60_min_con_1	con
SA339952	Donor28_stage1_60_min_con_1	con
SA339953	Donor28_stage1_120_min_con_1	con
SA339954	Donor28_stage1_60_min_con_2	con
SA339955	Donor27_stage1_60_min_con_1	con
SA339956	Donor27_stage1_0_min_con_2	con
SA339957	Donor31_stage1_120_min_con_1	con
SA339958	Donor31_stage1_120_min_con_2	con
SA339959	Donor31_stage1_60_min_con_2	con
SA339960	Donor31_stage1_60_min_con_1	con
SA339961	Donor31_stage1_0_min_con_1	con
SA339962	Donor31_stage1_0_min_con_2	con
SA339963	Donor31_stage1_240_min_con_1	con
SA339964	Donor31_stage1_240_min_con_2	con
SA339965	Donor26_stage1_0_min_con_1	con
SA339966	Donor32_stage1_0_min_con_1	con
SA339967	Donor26_stage1_0_min_con_2	con
SA339968	Donor26_stage1_60_min_con_1	con
SA339969	Donor31_stage1_480_min_con_1	con
SA339970	Donor31_stage1_480_min_con_2	con
SA339971	Donor26_stage1_60_min_con_2	con
SA339972	Donor26_stage1_120_min_con_1	con
SA339973	Donor30_stage1_120_min_con_2	con
SA339974	Donor30_stage1_240_min_con_1	con
SA339975	Donor30_stage1_120_min_con_1	con
SA339976	Donor30_stage1_60_min_con_2	con
SA339977	Donor27_stage1_0_min_con_1	con
SA339978	Donor30_stage1_60_min_con_1	con
SA339979	Donor30_stage1_240_min_con_2	con
SA339980	Donor30_stage1_480_min_con_1	con
SA339981	Donor26_stage1_240_min_con_1	con
SA339982	Donor26_stage1_120_min_con_2	con
SA339983	Donor26_stage1_240_min_con_2	con
SA339984	Donor26_stage1_480_min_con_2	con
SA339985	Donor30_stage1_480_min_con_2	con
SA339986	Donor32_stage1_0_min_con_2	con
SA339987	Donor19_stage1_0_min_con_1	con