Summary of Study ST003251

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002018. The data can be accessed directly via it's Project DOI: 10.21228/M8RV57 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003251
Study TitleTemperature change elicits lipidome adaptation in the simple organisms Mycoplasma mycoides and JCVI-syn3B
Study SummaryCell membranes mediate interactions between life and its environment, with lipids determining their properties. Understanding how cells adjust their lipidomes to tune membrane properties is crucial yet poorly defined due to the complexity of most organisms. We used quantitative shotgun lipidomics to study temperature adaptation in the simple organism Mycoplasma mycoides and the minimal cell, JCVI-syn3B. We show that lipid abundances follow a universal logarithmic distribution across eukaryotes and bacteria, with comparable degrees of lipid remodeling for adaptation regardless of lipidomic or organismal complexity. Lipid features analysis demonstrates head group-specific acyl chain remodeling as characteristic of lipidome adaptation; its deficiency in Syn3B is associated with impaired homeoviscous adaptation. Temporal analysis reveals a two-stage cold adaptation process: swift cholesterol and cardiolipin shifts followed by gradual acyl chain modifications. This work provides an in-depth analysis of lipidome adaptation in minimal cells, laying a foundation to probe the design principles of living membranes.
Institute
TU Dresden
Last NameSaenz
First NameJames
AddressTatzberg 41, Dresden, Saxony, 01307, Germany
Emailjpsaenz81@gmail.com
Phone(+49) 351 463 43066
Submit Date2024-05-30
Analysis Type DetailLC-MS
Release Date2024-06-12
Release Version1
James Saenz James Saenz
https://dx.doi.org/10.21228/M8RV57
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Bacteria; Subject species: Mycoplasma mycoides, Minimal cell JCVI-syn3B (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Temperature Time (hrs)
SA353531S3_30_0hMinimal cell JCVI-Syn3B 25 0
SA353532S3_30_1hMinimal cell JCVI-Syn3B 25 1
SA353533S3_30_2hMinimal cell JCVI-Syn3B 25 2
SA353534S3_30_24hMinimal cell JCVI-Syn3B 25 24
SA353535S3_30_4hMinimal cell JCVI-Syn3B 25 4
SA353536S3_30_8hMinimal cell JCVI-Syn3B 25 8
SA353537S3_30 (adapted)Minimal cell JCVI-Syn3B 30 -
SA353538S3_33.5 (adapted)Minimal cell JCVI-Syn3B 33.5 -
SA353539S3_37 (adapted)Minimal cell JCVI-Syn3B 37 -
SA353540S3_37 start (Temporal)Minimal cell JCVI-Syn3B 37 -0.25
SA353541S3_37_0hMinimal cell JCVI-Syn3B 37 24.25
SA353542S3_37_1hMinimal cell JCVI-Syn3B 37 25.25
SA353543S3_37_2hMinimal cell JCVI-Syn3B 37 26.25
SA353544S3_37_4hMinimal cell JCVI-Syn3B 37 28.25
SA353545S3_37_8hMinimal cell JCVI-Syn3B 37 32.25
SA353546S3_37_24hMinimal cell JCVI-Syn3B 37 48.25
SA353513MM_25 (adapted)M. mycoides 25 -
SA353514MM_25_0hM. mycoides 25 0
SA353515MM_25_1hM. mycoides 25 1
SA353516MM_25_2hM. mycoides 25 2
SA353517MM_25_24hM. mycoides 25 24
SA353518MM_25_4hM. mycoides 25 4
SA353519MM_25_8hM. mycoides 25 8
SA353520MM_27 (adapted)M. mycoides 27 -
SA353521MM_30 (adapted)M. mycoides 30 -
SA353522MM_33.5 (adapted)M. mycoides 33.5 -
SA353523MM_37 (adapted)M. mycoides 37 -
SA353524MM_37 start (Temporal)M. mycoides 37 -0.25
SA353525MM_37_0hM. mycoides 37 24.25
SA353526MM_37_1hM. mycoides 37 25.25
SA353527MM_37_2hM. mycoides 37 26.25
SA353528MM_37_4hM. mycoides 37 28.25
SA353529MM_37_8hM. mycoides 37 32.25
SA353530MM_37_24hM. mycoides 37 48.25
Showing results 1 to 34 of 34
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