Summary of Study ST003435

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001941. The data can be accessed directly via it's Project DOI: 10.21228/M8TM76 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003435
Study TitleMetabolomics analysis of zebrafish embryos treated with rotenone, Fasnall, TVB-2640, and GSK2194069
Study SummaryTo compare the metabolic effects and toxicity of Fasnall with rotenone in vivo, zebrafish embryos 48 h post-fertilization were exposed to a drug-containing medium for 6 h. Rotenone at 25 nM is lethal to fish embryos, while 5 nM concentration leads to a ~15-fold lactate accumulation. Similarly, Fasnall treatment increases lactate content, although the magnitude of the effect is significantly lower. Unlike 5 nM rotenone, Fasnall treatment does not cause visible phenotypic changes in the yolk. The zebrafish model suggests that Fasnall acts as a Complex I inhibitor in vivo.
Institute
Wistar Institute
DepartmentMolecular and Cellular Oncogenesis Program, Ellen and Ronald Caplan Cancer Center
LaboratorySchug's Lab
Last NameMukha
First NameDzmitry
Address3601 Spruce St, Philadelphia, PA 19104, USA
Emaildmukha@wistar.org
Phone+12154956903
Submit Date2024-08-21
Num Groups16
Total Subjects48
PublicationsSubmission Pending
Raw Data AvailableYes
Raw Data File Type(s)mzML, wiff
Analysis Type DetailLC-MS
Release Date2024-09-12
Release Version1
Dzmitry Mukha Dzmitry Mukha
https://dx.doi.org/10.21228/M8TM76
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Fish; Subject species: Danio rerio (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Sample Type Drug Treatment
SA37812524 BlankNA Blank NA
SA37812649 BlankNA Blank NA
SA37812701 BlankNA Blank NA
SA37812802 DMEM/F12 no addsNA QC NA
SA37812948 DMEM/F12 no addsNA QC NA
SA37813036 13-1 Rotenone 1 nM Medium 1Zebrafish medium Zebrafish medium 1 nM rotenone
SA37813138 13-3 Rotenone 1 nM Medium 3Zebrafish medium Zebrafish medium 1 nM rotenone
SA37813237 13-2 Rotenone 1 nM Medium 2Zebrafish medium Zebrafish medium 1 nM rotenone
SA37813343 21-2 Fasnall 20 uM Medium 2Zebrafish medium Zebrafish medium 20 uM Fasnall
SA37813444 21-3 Fasnall 20 uM Medium 3Zebrafish medium Zebrafish medium 20 uM Fasnall
SA37813542 21-1 Fasnall 20 uM Medium 1Zebrafish medium Zebrafish medium 20 uM Fasnall
SA37813645 25-1 Fasnall 40 uM Medium 1Zebrafish medium Zebrafish medium 40 uM Fasnall
SA37813746 25-2 Fasnall 40 uM Medium 2Zebrafish medium Zebrafish medium 40 uM Fasnall
SA37813847 25-3 Fasnall 40 uM Medium 3Zebrafish medium Zebrafish medium 40 uM Fasnall
SA37813931 05-2 GSK 40 uM Medium 2Zebrafish medium Zebrafish medium 40 uM GSK2194069
SA37814030 05-1 GSK 40 uM Medium 1Zebrafish medium Zebrafish medium 40 uM GSK2194069
SA37814132 05-3 GSK 40 uM Medium 3Zebrafish medium Zebrafish medium 40 uM GSK2194069
SA37814234 09-2 TVB-2640 40 uM Medium 2Zebrafish medium Zebrafish medium 40 uM TVB-2640
SA37814333 09-1 TVB-2640 40 uM Medium 1Zebrafish medium Zebrafish medium 40 uM TVB-2640
SA37814435 09-3 TVB-2640 40 uM Medium 3Zebrafish medium Zebrafish medium 40 uM TVB-2640
SA37814540 17-2 Rotenone 5 nM Medium 2Zebrafish medium Zebrafish medium 5 nM rotenone
SA37814641 17-3 Rotenone 5 nM Medium 3Zebrafish medium Zebrafish medium 5 nM rotenone
SA37814739 17-1 Rotenone 5 nM Medium 1Zebrafish medium Zebrafish medium 5 nM rotenone
SA37814829 01-3 DMSO Medium 3Zebrafish medium Zebrafish medium Vehicle
SA37814928 01-2 DMSO Medium 2Zebrafish medium Zebrafish medium Vehicle
SA37815027 01-1 DMSO Medium 1Zebrafish medium Zebrafish medium Vehicle
SA37815106 14 Rotenone 1 nM 1Zebrafish Zebrafish embryo metabolite extract 1 nM rotenone
SA37815220 16 Rotenone 1 nM 3 *Zebrafish Zebrafish embryo metabolite extract 1 nM rotenone
SA37815313 15 Rotenone 1 nM 2Zebrafish Zebrafish embryo metabolite extract 1 nM rotenone
SA37815422 24 Fasnall 20 uM 3Zebrafish Zebrafish embryo metabolite extract 20 uM Fasnall
SA37815515 23 Fasnall 20 uM 2Zebrafish Zebrafish embryo metabolite extract 20 uM Fasnall
SA37815608 22 Fasnall 20 uM 1Zebrafish Zebrafish embryo metabolite extract 20 uM Fasnall
SA37815716 27 Fasnall 40 uM 2Zebrafish Zebrafish embryo metabolite extract 40 uM Fasnall
SA37815809 26 Fasnall 40 uM 1Zebrafish Zebrafish embryo metabolite extract 40 uM Fasnall
SA37815923 28 Fasnall 40 uM 3Zebrafish Zebrafish embryo metabolite extract 40 uM Fasnall
SA37816025 08 GSK 40 uM 3Zebrafish Zebrafish embryo metabolite extract 40 uM GSK2194069
SA37816104 06 GSK 40 uM 1Zebrafish Zebrafish embryo metabolite extract 40 uM GSK2194069
SA37816218 07 GSK 40 uM 2Zebrafish Zebrafish embryo metabolite extract 40 uM GSK2194069
SA37816311 07 GSK 40 uM 2Zebrafish Zebrafish embryo metabolite extract 40 uM GSK2194069
SA37816405 10 TVB-2640 40 uM 1Zebrafish Zebrafish embryo metabolite extract 40 uM TVB-2640
SA37816519 12 TVB-2640 40 uM 3Zebrafish Zebrafish embryo metabolite extract 40 uM TVB-2640
SA37816612 11 TVB-2640 40 uM 2Zebrafish Zebrafish embryo metabolite extract 40 uM TVB-2640
SA37816721 19 Rotenone 5 nM 2Zebrafish Zebrafish embryo metabolite extract 5 nM rotenone
SA37816814 19 Rotenone 5 nM 2Zebrafish Zebrafish embryo metabolite extract 5 nM rotenone
SA37816907 18 Rotenone 5 nM 1Zebrafish Zebrafish embryo metabolite extract 5 nM rotenone
SA37817026 20 Rotenone 5 nM 3Zebrafish Zebrafish embryo metabolite extract 5 nM rotenone
SA37817117 04 DMSO 3Zebrafish Zebrafish embryo metabolite extract Vehicle
SA37817210 03 DMSO 2Zebrafish Zebrafish embryo metabolite extract Vehicle
SA37817303 02 DMSO 1Zebrafish Zebrafish embryo metabolite extract Vehicle
Showing results 1 to 49 of 49
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