Summary of Study ST003435
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001941. The data can be accessed directly via it's Project DOI: 10.21228/M8TM76 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003435 |
Study Title | Metabolomics analysis of zebrafish embryos treated with rotenone, Fasnall, TVB-2640, and GSK2194069 |
Study Summary | To compare the metabolic effects and toxicity of Fasnall with rotenone in vivo, zebrafish embryos 48 h post-fertilization were exposed to a drug-containing medium for 6 h. Rotenone at 25 nM is lethal to fish embryos, while 5 nM concentration leads to a ~15-fold lactate accumulation. Similarly, Fasnall treatment increases lactate content, although the magnitude of the effect is significantly lower. Unlike 5 nM rotenone, Fasnall treatment does not cause visible phenotypic changes in the yolk. The zebrafish model suggests that Fasnall acts as a Complex I inhibitor in vivo. |
Institute | Wistar Institute |
Department | Molecular and Cellular Oncogenesis Program, Ellen and Ronald Caplan Cancer Center |
Laboratory | Schug's Lab |
Last Name | Mukha |
First Name | Dzmitry |
Address | 3601 Spruce St, Philadelphia, PA 19104, USA |
dmukha@wistar.org | |
Phone | +12154956903 |
Submit Date | 2024-08-21 |
Num Groups | 16 |
Total Subjects | 48 |
Publications | Submission Pending |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML, wiff |
Analysis Type Detail | LC-MS |
Release Date | 2024-09-12 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Fish; Subject species: Danio rerio (Factor headings shown in green)
mb_sample_id | local_sample_id | Sample source | Sample Type | Drug Treatment |
---|---|---|---|---|
SA378125 | 24 Blank | NA | Blank | NA |
SA378126 | 49 Blank | NA | Blank | NA |
SA378127 | 01 Blank | NA | Blank | NA |
SA378128 | 02 DMEM/F12 no adds | NA | QC | NA |
SA378129 | 48 DMEM/F12 no adds | NA | QC | NA |
SA378130 | 36 13-1 Rotenone 1 nM Medium 1 | Zebrafish medium | Zebrafish medium | 1 nM rotenone |
SA378131 | 38 13-3 Rotenone 1 nM Medium 3 | Zebrafish medium | Zebrafish medium | 1 nM rotenone |
SA378132 | 37 13-2 Rotenone 1 nM Medium 2 | Zebrafish medium | Zebrafish medium | 1 nM rotenone |
SA378133 | 43 21-2 Fasnall 20 uM Medium 2 | Zebrafish medium | Zebrafish medium | 20 uM Fasnall |
SA378134 | 44 21-3 Fasnall 20 uM Medium 3 | Zebrafish medium | Zebrafish medium | 20 uM Fasnall |
SA378135 | 42 21-1 Fasnall 20 uM Medium 1 | Zebrafish medium | Zebrafish medium | 20 uM Fasnall |
SA378136 | 45 25-1 Fasnall 40 uM Medium 1 | Zebrafish medium | Zebrafish medium | 40 uM Fasnall |
SA378137 | 46 25-2 Fasnall 40 uM Medium 2 | Zebrafish medium | Zebrafish medium | 40 uM Fasnall |
SA378138 | 47 25-3 Fasnall 40 uM Medium 3 | Zebrafish medium | Zebrafish medium | 40 uM Fasnall |
SA378139 | 31 05-2 GSK 40 uM Medium 2 | Zebrafish medium | Zebrafish medium | 40 uM GSK2194069 |
SA378140 | 30 05-1 GSK 40 uM Medium 1 | Zebrafish medium | Zebrafish medium | 40 uM GSK2194069 |
SA378141 | 32 05-3 GSK 40 uM Medium 3 | Zebrafish medium | Zebrafish medium | 40 uM GSK2194069 |
SA378142 | 34 09-2 TVB-2640 40 uM Medium 2 | Zebrafish medium | Zebrafish medium | 40 uM TVB-2640 |
SA378143 | 33 09-1 TVB-2640 40 uM Medium 1 | Zebrafish medium | Zebrafish medium | 40 uM TVB-2640 |
SA378144 | 35 09-3 TVB-2640 40 uM Medium 3 | Zebrafish medium | Zebrafish medium | 40 uM TVB-2640 |
SA378145 | 40 17-2 Rotenone 5 nM Medium 2 | Zebrafish medium | Zebrafish medium | 5 nM rotenone |
SA378146 | 41 17-3 Rotenone 5 nM Medium 3 | Zebrafish medium | Zebrafish medium | 5 nM rotenone |
SA378147 | 39 17-1 Rotenone 5 nM Medium 1 | Zebrafish medium | Zebrafish medium | 5 nM rotenone |
SA378148 | 29 01-3 DMSO Medium 3 | Zebrafish medium | Zebrafish medium | Vehicle |
SA378149 | 28 01-2 DMSO Medium 2 | Zebrafish medium | Zebrafish medium | Vehicle |
SA378150 | 27 01-1 DMSO Medium 1 | Zebrafish medium | Zebrafish medium | Vehicle |
SA378151 | 06 14 Rotenone 1 nM 1 | Zebrafish | Zebrafish embryo metabolite extract | 1 nM rotenone |
SA378152 | 20 16 Rotenone 1 nM 3 * | Zebrafish | Zebrafish embryo metabolite extract | 1 nM rotenone |
SA378153 | 13 15 Rotenone 1 nM 2 | Zebrafish | Zebrafish embryo metabolite extract | 1 nM rotenone |
SA378154 | 22 24 Fasnall 20 uM 3 | Zebrafish | Zebrafish embryo metabolite extract | 20 uM Fasnall |
SA378155 | 15 23 Fasnall 20 uM 2 | Zebrafish | Zebrafish embryo metabolite extract | 20 uM Fasnall |
SA378156 | 08 22 Fasnall 20 uM 1 | Zebrafish | Zebrafish embryo metabolite extract | 20 uM Fasnall |
SA378157 | 16 27 Fasnall 40 uM 2 | Zebrafish | Zebrafish embryo metabolite extract | 40 uM Fasnall |
SA378158 | 09 26 Fasnall 40 uM 1 | Zebrafish | Zebrafish embryo metabolite extract | 40 uM Fasnall |
SA378159 | 23 28 Fasnall 40 uM 3 | Zebrafish | Zebrafish embryo metabolite extract | 40 uM Fasnall |
SA378160 | 25 08 GSK 40 uM 3 | Zebrafish | Zebrafish embryo metabolite extract | 40 uM GSK2194069 |
SA378161 | 04 06 GSK 40 uM 1 | Zebrafish | Zebrafish embryo metabolite extract | 40 uM GSK2194069 |
SA378162 | 18 07 GSK 40 uM 2 | Zebrafish | Zebrafish embryo metabolite extract | 40 uM GSK2194069 |
SA378163 | 11 07 GSK 40 uM 2 | Zebrafish | Zebrafish embryo metabolite extract | 40 uM GSK2194069 |
SA378164 | 05 10 TVB-2640 40 uM 1 | Zebrafish | Zebrafish embryo metabolite extract | 40 uM TVB-2640 |
SA378165 | 19 12 TVB-2640 40 uM 3 | Zebrafish | Zebrafish embryo metabolite extract | 40 uM TVB-2640 |
SA378166 | 12 11 TVB-2640 40 uM 2 | Zebrafish | Zebrafish embryo metabolite extract | 40 uM TVB-2640 |
SA378167 | 21 19 Rotenone 5 nM 2 | Zebrafish | Zebrafish embryo metabolite extract | 5 nM rotenone |
SA378168 | 14 19 Rotenone 5 nM 2 | Zebrafish | Zebrafish embryo metabolite extract | 5 nM rotenone |
SA378169 | 07 18 Rotenone 5 nM 1 | Zebrafish | Zebrafish embryo metabolite extract | 5 nM rotenone |
SA378170 | 26 20 Rotenone 5 nM 3 | Zebrafish | Zebrafish embryo metabolite extract | 5 nM rotenone |
SA378171 | 17 04 DMSO 3 | Zebrafish | Zebrafish embryo metabolite extract | Vehicle |
SA378172 | 10 03 DMSO 2 | Zebrafish | Zebrafish embryo metabolite extract | Vehicle |
SA378173 | 03 02 DMSO 1 | Zebrafish | Zebrafish embryo metabolite extract | Vehicle |
Showing results 1 to 49 of 49 |