Summary of Study ST003623

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002208. The data can be accessed directly via it's Project DOI: 10.21228/M86Z6P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003623
Study TitleNRF2 supports non-small cell lung cancer growth independently of CBP/p300-enhanced glutathione synthesis: Global metabolomics analysis on A549 cells at different NRF2 status (Part 1 of 3)
Study TypeUntargeted Metabolomics
Study SummaryThis study aims at discovering metabolic changes in A549 cancer cells in the presence and absence of NRF2, and a mutant NRF2 genotypes. Metabolites were extracted from cell pellets by using an LLE method with Methanol/Chloroform/water. The aqueous layer was analyzed by HILIC-HRMS. An in-house RT library was used to identify metabolites. Statistical analyses was performed to identify statistically significant changes in the metabolism. 35 metabolites presented differential abundance between NRF2 knockdown and wildtype conditions. In particular, GSH and several glutamate dipeptides were significantly depleted upon NRF2 knockdown, in line with the prevailing role of NRF2 in controlling GSH biosynthesis. Disruption of additional metabolites involved in the PPP (sedoheptulose-7-phosphate, S7P), nucleotide (CMP, IMP) and amino acid (kynurenine, homocitrulline) metabolism were also observed upon NRF2 knockdown.
Institute
Genentech Inc.
Last NameWang
First NameMike
Address1 DNA Way, South San Francisco, CA 94080, USA
Emailwang.mike@gene.com
Phone6502457991
Submit Date2024-12-06
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2025-01-02
Release Version1
Mike Wang Mike Wang
https://dx.doi.org/10.21228/M86Z6P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Genotype Treatment Sample Label
SA393310Sample21A549 human lung epithelial cells shNRF2-Ful length NRF2 Dox+ FL+1
SA393314Sample05A549 human lung epithelial cells shNRF2-Ful length NRF2 Dox- FL-1
SA393311Sample22A549 human lung epithelial cells shNRF2-Ful length NRF2 Dox+ FL+2
SA393315Sample06A549 human lung epithelial cells shNRF2-Ful length NRF2 Dox- FL-2
SA393312Sample23A549 human lung epithelial cells shNRF2-Ful length NRF2 Dox+ FL+3
SA393316Sample07A549 human lung epithelial cells shNRF2-Ful length NRF2 Dox- FL-3
SA393313Sample24A549 human lung epithelial cells shNRF2-Ful length NRF2 Dox+ FL+4
SA393317Sample08A549 human lung epithelial cells shNRF2-Ful length NRF2 Dox- FL-4
SA393318Sample41A549 human lung epithelial cells shNRF2-Luciferase Dox+A485 A485+1
SA393319Sample42A549 human lung epithelial cells shNRF2-Luciferase Dox+A485 A485+2
SA393320Sample43A549 human lung epithelial cells shNRF2-Luciferase Dox+A485 A485+3
SA393321Sample44A549 human lung epithelial cells shNRF2-Luciferase Dox+A485 A485+4
SA393322Sample33A549 human lung epithelial cells shNRF2-Luciferase Dox+DMSO DMSO+1
SA393323Sample34A549 human lung epithelial cells shNRF2-Luciferase Dox+DMSO DMSO+2
SA393324Sample35A549 human lung epithelial cells shNRF2-Luciferase Dox+DMSO DMSO+3
SA393325Sample36A549 human lung epithelial cells shNRF2-Luciferase Dox+DMSO DMSO+4
SA393326Sample17A549 human lung epithelial cells shNRF2-Luciferase Dox+ luc+1
SA393330Sample01A549 human lung epithelial cells shNRF2-Luciferase Dox- luc-1
SA393327Sample18A549 human lung epithelial cells shNRF2-Luciferase Dox+ luc+2
SA393331Sample02A549 human lung epithelial cells shNRF2-Luciferase Dox- luc-2
SA393328Sample19A549 human lung epithelial cells shNRF2-Luciferase Dox+ luc+3
SA393332Sample03A549 human lung epithelial cells shNRF2-Luciferase Dox- luc-3
SA393329Sample20A549 human lung epithelial cells shNRF2-Luciferase Dox+ luc+4
SA393333Sample04A549 human lung epithelial cells shNRF2-Luciferase Dox- luc-4
SA393334Sample25A549 human lung epithelial cells shNRF2-NRF2 Neh4,5 mut Dox+ 45+1
SA393338Sample09A549 human lung epithelial cells shNRF2-NRF2 Neh4,5 mut Dox- 45-1
SA393335Sample26A549 human lung epithelial cells shNRF2-NRF2 Neh4,5 mut Dox+ 45+2
SA393339Sample10A549 human lung epithelial cells shNRF2-NRF2 Neh4,5 mut Dox- 45-2
SA393336Sample27A549 human lung epithelial cells shNRF2-NRF2 Neh4,5 mut Dox+ 45+3
SA393340Sample11A549 human lung epithelial cells shNRF2-NRF2 Neh4,5 mut Dox- 45-3
SA393337Sample28A549 human lung epithelial cells shNRF2-NRF2 Neh4,5 mut Dox+ 45+4
SA393341Sample12A549 human lung epithelial cells shNRF2-NRF2 Neh4,5 mut Dox- 45-4
Showing results 1 to 32 of 32
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