Summary of Study ST003643

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002253. The data can be accessed directly via it's Project DOI: 10.21228/M8DC25 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003643
Study TitleMetabolite analysis for WT and BnaMYB52 mutants by LC-MS/MS
Study SummaryBnaA09.MYB52 directly targets the BnaBAN promoters and promotes BnaBAN expression in Brassica napus. BAN, encoding anthocyanidin reductase that converts anthocyanidins to 2,3- cis-flavan-3-ols compounds (proanthocyanidins starter units), is involved in the flavonoid biosynthesis pathway. Thus, Metabolite analysis was conducted to detect the content of flavonoid in WT (Wild-type), OE (BnaA09.MYB52 overexpression lines in the genetic background Westar) and mutants (four homologous genes of BnaMYB52 knocked out) plants. About 0.1 g mature seeds were collected from WT, OE and mutant plants. Metabolites analysis demonstrated that BnaMYB52 positively regulated the content of several metabolites (such as L-phenylalanine, p-coumaric acid, grosvenorine and astragalin) in flavonoid pathway.
Institute
Huazhong Agricultural University
Last NameJiang
First NameYe
AddressShi Zishan Street 1th, Wuhan, Hubei, 430070, China
Emailvyejiang@163.com
Phone13697353446
Submit Date2024-12-21
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2025-01-26
Release Version1
Ye Jiang Ye Jiang
https://dx.doi.org/10.21228/M8DC25
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Plant; Subject species: Brassica napus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA39782952CR-1-4Mutant
SA39783052CR-2-4Mutant
SA39783152CR-2-2Mutant
SA39783252CR-2-1Mutant
SA39783352CR-2-3Mutant
SA39783452CR-1-3Mutant
SA39783552CR-1-2Mutant
SA39783652CR-1-1Mutant
SA39783752OE-1-1OE
SA39783852OE-1-2OE
SA39783952OE-1-3OE
SA39784052OE-1-4OE
SA39784152OE-2-1OE
SA39784252OE-2-2OE
SA39784352OE-2-3OE
SA397844WT-4Wide-type
SA397845WT-2Wide-type
SA397846WT-3Wide-type
SA397847WT-1Wide-type
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