Summary of Study ST003790

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002366. The data can be accessed directly via it's Project DOI: 10.21228/M8SZ6Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003790
Study TitleFecal metabolomics of B16-OVA tumor-bearing mice fed chow or low and high fiber purified diets and treated with isotype control or anti-PD-1 antibody
Study SummaryImmune checkpoint blockade (ICB) has transformed cancer treatment, but success rates remain limited. Recent research suggests dietary fiber enhances ICB response in mice and patients through microbiome-dependent mechanisms. Yet, the robustness of this effect across cancer types and dietary contexts remains unclear. Specifically, prior literature compared grain-based chow (high fiber) to low-fiber purified diet, but these diets differ also on other dimensions including phytochemicals. Here we investigated, in mice fed grain-based chow or purified diets with differing quantities of isolated fibers (cellulose and inulin), the gut microbiome, metabolite levels and ICB activity in multiple tumor models. Isolated fibers shifted multiple microbial taxa toward chow-like levels, although diet-type (chow vs. purified) had a stronger impact on gut-microbiome composition. Metabolomic profiles were relatively similar between mice fed high- and low-fiber purified diets, but differed massively between mice fed purified diets or chow, identifying the factor as diet type, independent of fiber. Tumor growth studies in multiple murine models revealed that fiber has a weaker impact on ICB (anti-PD-1) efficacy than previously reported. While diet impacted ICB activity in some models, the effect was not directionally consistent. In no model did we observe the pattern expected if fiber controlled ICB efficacy: strong efficacy in both chow and high-fiber purified diet but low efficacy in low-fiber purified diet. Thus, dietary fiber appears to have limited or inconsistent effect on ICB efficacy in mouse models, and other dietary factors that correlate with fiber intake may underlie the clinical correlations between fiber consumption and immunotherapy outcomes.
Institute
Princeton University
Last NameRoichman
First NameAsael
AddressLewis-Sigler Institute for Integrative Genomics Princeton University Carl Icahn Laboratory, Washington Road Princeton, NJ 08544
Emailasael100@gmail.com
Phone9084101710
Submit Date2025-03-11
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2025-03-31
Release Version1
Asael Roichman Asael Roichman
https://dx.doi.org/10.21228/M8SZ6Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Diet Treatment
SA412545blank2blank blank blank
SA412546blank1blank blank blank
SA41250959Feces Chow anti-PD-1
SA41251064Feces Chow anti-PD-1
SA41251163Feces Chow anti-PD-1
SA41251261Feces Chow anti-PD-1
SA41251360Feces Chow anti-PD-1
SA41251462Feces Chow anti-PD-1
SA41251556Feces Chow isotype control
SA41251655Feces Chow isotype control
SA41251754Feces Chow isotype control
SA41251853Feces Chow isotype control
SA41251952Feces Chow isotype control
SA41252051Feces Chow isotype control
SA41252176Feces HC_In0 anti-PD-1
SA41252278Feces HC_In0 anti-PD-1
SA41252377Feces HC_In0 anti-PD-1
SA41252473Feces HC_In0 anti-PD-1
SA41252574Feces HC_In0 anti-PD-1
SA41252675Feces HC_In0 anti-PD-1
SA41252785Feces HC_In1.9 anti-PD-1
SA41252884Feces HC_In1.9 anti-PD-1
SA41252983Feces HC_In1.9 anti-PD-1
SA41253082Feces HC_In1.9 anti-PD-1
SA41253186Feces HC_In1.9 anti-PD-1
SA41253287Feces HC_In1.9 anti-PD-1
SA41253389Feces HC_In8 anti-PD-1
SA41253490Feces HC_In8 anti-PD-1
SA41253592Feces HC_In8 anti-PD-1
SA41253693Feces HC_In8 anti-PD-1
SA41253795Feces HC_In8 anti-PD-1
SA41253894Feces HC_In8 anti-PD-1
SA41253972Feces LC_In0 anti-PD-1
SA41254071Feces LC_In0 anti-PD-1
SA41254168Feces LC_In0 anti-PD-1
SA41254267Feces LC_In0 anti-PD-1
SA41254366Feces LC_In0 anti-PD-1
SA41254465Feces LC_In0 anti-PD-1
Showing results 1 to 38 of 38
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