Summary of Study ST004231

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002669. The data can be accessed directly via it's Project DOI: 10.21228/M8NN9R This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST004231
Study TitleMetabolomic studies of H9c2 cells samples
Study SummaryCardiomyocyte differentiation is a complex process involving significant metabolic remodeling, but its impact on cellular redox state and cell damage remains poorly understood. Using metabolomic, biophysical, and biochemical approaches, we characterized, in vitro, the metabolic shift of differentiating cardiomyocytes and its implications for oxidative damage. We found that differentiating cardiomyocytes undergo a broad metabolic reprogramming from a glycolytic to an oxidative state, marked by increased activity in key pathways, including malate-aspartate shuttle, glutathione metabolism, and tricarboxylic acid cycle. This metabolic transition was associated with mitochondrial enlargement and increased reactive oxygen species (ROS) production. Intriguingly, despite ROS increase, differentiated cells maintained similar levels of DNA damage as cardiomyoblasts and were more resistant to a H₂O₂ challenge. Our findings suggest that metabolic adaptations during cardiomyocyte differentiation enhance their capacity to mitigate oxidative stress damage, providing an adaptive avenue that enables cardiomyocyte survival upon exposure to a rich oxygen environment.
Institute
University of Campinas
Last NameAmaral
First NameAlan
AddressRua Josué de Castro, s/n - Cidade Universitária, Campinas - SP, 13083-970
Emaila228197@dac.unicamp.br
Phone+5527996413870
Submit Date2025-09-16
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2025-09-29
Release Version1
Alan Amaral Alan Amaral
https://dx.doi.org/10.21228/M8NN9R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Time
SA486178ENDO_01H9c2 cells T0
SA486179ENDO_02H9c2 cells T0
SA486180ENDO_03H9c2 cells T0
SA486181ENDO_04H9c2 cells T03
SA486182ENDO_05H9c2 cells T03
SA486183ENDO_06H9c2 cells T03
SA486184ENDO_07H9c2 cells T05
SA486185ENDO_08H9c2 cells T05
SA486186ENDO_09H9c2 cells T05
SA486187ENDO_10H9c2 cells T07
SA486188ENDO_11H9c2 cells T07
SA486189ENDO_12H9c2 cells T07
SA486190ENDO_13H9c2 cells T10
SA486191ENDO_14H9c2 cells T10
SA486192ENDO_15H9c2 cells T10
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