Summary of study ST000107

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000046. The data can be accessed directly via it's Project DOI: 10.21228/M84S36 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000107
Study TitleMetabolomic and lipidomic profiles in response to exogenous insulin and GLP-1 infusions during prolonged fasting
Study TypeTimecourse
Study SummarySeventeen northern elephant seal pups constituting four different cohorts at Año Nuevo State Reserve were studied at two post-weaning periods: early (1-2 wk post weaning; n=5) and late (6-7 weeks post weaning; n=12). Study #1: Prior to each infusion protocol, plasma U/kg). Following the infusion, blood samples were collected at 10,To determine the effects of prolonged fasting on peripheral insulin activity and function, samples were collected. Ten fasting seal pups (n=5 early, n=5 late) were infused (i.v.) with a mass-specific dose of insulin (0.065 U/kg). Following the infusion, blood samples were collected at 10, 30, 60, 90, and 120 minutes. Study #2: Seven, late-fasted seal pups were administered either a low (LDG; 10 pmol/kg; n=3) or high (HDG; 100 pmol/kg; n=4) dose of GLP-1 immediately following a glucose bolus (0.5 g/kg) (i.v.) infused within 2 mins. the infusions, blood samples were collected at 10, 30, 60, 90, 120, and 150 minutes.
Institute
University of California, Davis
DepartmentNutrition
LaboratoryNewman
Last NameNewman
First NameJohn
Address430 W. Health Sciences Dr., Davis, CA 95616
Emailjohn.newman@ars.usda.gov
Phone+1-530-752-1009
Submit Date2014-07-10
Num Groups4
Total Subjects5
Raw Data AvailableYes
Raw Data File Type(s)mzML, .MS, .txt, INI
Uploaded File Size751 M
Analysis Type DetailGC/LC-MS
Release Date2015-02-03
Release Version1
John Newman John Newman
https://dx.doi.org/10.21228/M84S36
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000177 AN000178
Analysis type MS MS
Chromatography type GC GC
Chromatography system
Column
MS Type ESI EI
MS instrument type Triple quadrupole Single quadrupole
MS instrument name ABI Sciex API 4000 QTrap Agilent 5973N
Ion Mode POSITIVE POSITIVE
Units nM nM

MS:

MS ID:MS000140
Analysis ID:AN000177
Instrument Name:ABI Sciex API 4000 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:LC/MS
Ion Mode:POSITIVE
Ion Source Temperature:See protocol/methods file
Ion Spray Voltage:See protocol/methods file
  
MS ID:MS000141
Analysis ID:AN000178
Instrument Name:Agilent 5973N
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:LC/MS
Ion Mode:POSITIVE
Ion Source Temperature:See protocol/methods file
Ion Spray Voltage:See protocol/methods file
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