Summary of Study ST000275
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000219. The data can be accessed directly via it's Project DOI: 10.21228/M8359Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000275 |
| Study Title | Metabolic analysis of Parp1 ko/wt Saline & Bleo Mouse Lung Fiboblasts and Human IPF & Normal Lung Fiboblasts (Part 2) |
| Study Type | Glycolysis/TCA/Nucleotide analysis. Ceramide analysis for parp1 wild type lung tissue/Cells after saline or bleomycin treatment. |
| Study Summary | This study is a part of series performed for the same researcher through pilot/feasibility grant program, so the publication is relevant reference for other studies (ST000143, ST000183) This specific experiment is a small pilot study to establish method performance, it includes four biological replicas of identical cell cultures after the identical treatment and a single tissue sample. |
| Institute | University of Michigan |
| Department | Deaprtment of Pathology |
| Laboratory | Sem H. Phan |
| Last Name | Hu |
| First Name | Biao |
| Address | Ann Arbor, MI |
| biaohu@med.umich.edu | |
| Phone | 734-7635731 |
| Submit Date | 2014-06-11 |
| Num Groups | 1 |
| Total Subjects | 5 |
| Study Comments | http://www.atsjournals.org/doi/full/10.1165/rcmb.2014-0108OC#.Vmb9VVWrRhE |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-01-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000438 | AN000439 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1260 | Agilent 1260 |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | µM (500µl of extraction solvent) |
MS:
| MS ID: | MS000379 |
| Analysis ID: | AN000438 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | Column1_solv1_jetstream+_grad9.m |
| Processing Parameters File: | EX00310-MassHunterQuant-GlyTCA-DataAnalysis-LCMS-Method.m |
| MS ID: | MS000380 |
| Analysis ID: | AN000439 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | ALPHA_KETO_ACIDS-FULL.M |
| Processing Parameters File: | EX00310-MassHunterQuant-GlyTCA-DataAnalysis-GCMS-Method.m |
