Summary of Study ST000980
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000670. The data can be accessed directly via it's Project DOI: 10.21228/M82Q3W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000980 |
| Study Title | Metabolomic Analysis of plasma samples from Non-Allergic Subjects and Profilin-Allergic Patients overexposed to Grass Pollen |
| Study Type | Allergy severity comparison |
| Study Summary | Prevalence and severity of allergic diseases have increased worldwide. To date, respiratory allergy phenotypes are not fully characterized and, along with inflammation progression, treatment is increasingly complex and expensive. Profilin sensitization constitutes a good model to study the progression of allergic inflammation. Our aim was to identify the underlying mechanisms and the associated biomarkers of this progression, focusing on severe phenotypes, using transcriptomics and metabolomics. Methods: 25 subjects were included in the study. Plasma samples were analyzed using Gas and Liquid Chromatography coupled to Mass Spectrometry (GC-MS and LC-MS, respectively). Individuals were classified in 4 groups – “non-allergic”, “mild”, “moderate” and “severe” – based on their clinical history, their response to an oral challenge test with profilin, and after a refinement using a mathematical metabolomic model. PBMCs were used for microarray analysis. |
| Institute | The Centre of Metabolomics and Bioanalysis |
| Department | Analytical chemistry |
| Last Name | Obeso Montero |
| First Name | David |
| Address | Avda. Monteprincipe s/n 28668 Boadilla del Monte, Madrid, España |
| david.obesomontero@beca.ceu.es | |
| Phone | 913724769 |
| Submit Date | 2018-06-13 |
| Num Groups | 4 groups |
| Total Subjects | 25 plasma samples |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2018-07-17 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001604 | AN001605 | AN001606 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Normal phase | Normal phase | GC |
| Chromatography system | Agilent 1200 | Agilent 1200 | Agilent 7890A |
| Column | Unspecified | Unspecified | Unspecified |
| MS Type | ESI | ESI | EI |
| MS instrument type | QTOF | QTOF | Single quadrupole |
| MS instrument name | Agilent 6520 QTOF | Agilent 6520 QTOF | Agilent 5975C |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | peak area | peak area | Peak area |
MS:
| MS ID: | MS001482 |
| Analysis ID: | AN001604 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 3500 V |
| Dry Gas Flow: | 10.5 L/min |
| Dry Gas Temp: | 330 °C |
| Fragment Voltage: | 175 V |
| MS ID: | MS001483 |
| Analysis ID: | AN001605 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Capillary Voltage: | 4000 V |
| Dry Gas Flow: | 10.5 L/min |
| Dry Gas Temp: | 331 °C |
| Fragment Voltage: | 176 V |
| MS ID: | MS001484 |
| Analysis ID: | AN001606 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Helium Flow: | 1 mL/min |
| Ionization Energy: | -70 eV |
