Summary of Study ST001050

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000703. The data can be accessed directly via it's Project DOI: 10.21228/M8T10P This work is supported by NIH grant, U2C- DK119886.

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Study IDST001050
Study TitleP4HA1 knockdown in the breast cell line MDA231 Gln metabolism (part II)
Study Typeisotope tracer
Study SummaryDetermine the effect on glutamine metabolism of knocking down P4HA1 in the MDA231 cell line.
Institute
University of Kentucky
DepartmentMarkey Cancer Center
Last NameXiong
First NameGaofeng
AddressBBSRC361, 741 South Limestone, Lexington, KY 40536, USA
Emailgaofeng.xiong@uky.edu
Phone000-000-0000
Submit Date2018-09-12
Study CommentsFor additional comments about the study, like listing a publication.
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailGC-MS
Release Date2019-03-06
Release Version1
Gaofeng Xiong Gaofeng Xiong
https://dx.doi.org/10.21228/M8T10P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001865
Analysis type MS
Chromatography type GC
Chromatography system GC-EI-MS, Thermo ITQ
Column SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
MS Type EI
MS instrument type GC-ITQ
MS instrument name Thermo ITQ
Ion Mode POSITIVE
Units normalized peak area

MS:

MS ID:MS001725
Analysis ID:AN001865
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
MS Comments:GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide).
Ion Mode:POSITIVE
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