Summary of Study ST001133

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000758. The data can be accessed directly via it's Project DOI: 10.21228/M8Q10R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001133
Study TitleDownregulation of CENPF epigenetically remodels prostate cancer cells and alters cellular metabolism
Study SummaryThis study aimed to determine the metabolic profile of CENPF-knockout (CENPFKO) PC cells and identify differentially expressed metabolites (DEMs) that can be used as diagnostic markers.
Institute
Cedars-Sinai Medical Center
Last NameKim
First NameJayoung
Address8700 Beverly Blvd., Davis 5071 Los Angeles, CA 90048, USA
EmailJayoung.Kim@cshs.org
Phone310-423-7168
Submit Date2019-01-14
Analysis Type DetailGC-MS
Release Date2020-01-06
Release Version1
Jayoung Kim Jayoung Kim
https://dx.doi.org/10.21228/M8Q10R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001858
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890N
Column Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type GC x GC-TOF
MS instrument name Leco Pegasus IV TOF
Ion Mode POSITIVE
Units normalized intensity

MS:

MS ID:MS001718
Analysis ID:AN001858
Instrument Name:Leco Pegasus IV TOF
Instrument Type:GC x GC-TOF
MS Type:EI
MS Comments:Detection or deconvolution of peaks and compounds was performed using the Leco ChromaTOF software. Spectra were matched against the FiehnLib Mass Spectral and Retention Index Library.Post-curation and peak replacements were done using the in-house developed BinBase software, which was set as follows: validity of chromatogram (<10 peaks with intensities >107 counts/sec), unbiased retention index marker detection (MS similarity >800, validity of intensity range for high m/z marker ions), and retention index calculation by 5th order polynomial regression.
Ion Mode:POSITIVE
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