Summary of Study ST001167
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000779. The data can be accessed directly via it's Project DOI: 10.21228/M8097N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001167 |
| Study Title | Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry |
| Study Summary | We developed a stable-isotope tracing capillary electrophoresis (CE)-MS metabolomics approach to cover polar metabolites as well as isotopologues in a non-targeted way. An in-house developed software enables high throughput processing of complex multi-dimensional data. The practicability is demonstrated analysing 13C-U-glucose exposed prostate cancer and non-cancer cells. |
| Institute | Dalian Institute Of Chemical Physics |
| Last Name | Wang |
| First Name | Zhichao |
| Address | 457, Zhongshan Road |
| wangzc05@dicp.ac.cn | |
| Phone | +86-15998625250 |
| Submit Date | 2019-01-06 |
| Study Comments | Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry - A Novel Tool Complementing Metabolomics Analyses of Polar Metabolites |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-01-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001929 | AN001930 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | a fused silica capillary (50 μm i.d. × 80 cm) | a fused silica capillary (50 μm i.d. × 80 cm) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6224 TOF | Agilent 6224 TOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Relative intensity after normalization | Relative intensity after normalization |
MS:
| MS ID: | MS001785 |
| Analysis ID: | AN001929 |
| Instrument Name: | Agilent 6224 TOF |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | nebulizer pressure of 5 psig, dry gas temperature of 300 °C, nitrogen flow of 7 L/min, capillary voltage of 4 kV, fragmentor of 105 V, skimmer of 50 V, Oct RFV of 650 V, acquisition rate of 1.5 spectra/s and mass range of 60-1,000 were utilized. |
| Ion Mode: | POSITIVE |
| MS ID: | MS001786 |
| Analysis ID: | AN001930 |
| Instrument Name: | Agilent 6224 TOF |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | capillary voltage of 3.5 kV, fragmentor of 125 V and mass range of 50-1,000 were set in the TOF/MS analysis. During sample analysis, reference masses were applied to ensure the real-time calibration of exact mass measurement. |
| Ion Mode: | NEGATIVE |
