Summary of Study ST001288
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000870. The data can be accessed directly via it's Project DOI: 10.21228/M8796F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001288 |
| Study Title | Subcellular organelle lipidomics in TLR-4-activated macrophages |
| Study Summary | Lipids orchestrate biological processes by acting remotely as signaling molecules or locally as membrane components that modulate protein function. Detailed insight into lipid function requires knowledge of the subcellular localization of individual lipids. We report an analysis of the subcellular lipidome of the mammalian macrophage, a cell type that plays key roles in inflammation, immune responses, and phagocytosis. Nuclei, mitochondria, endoplasmic reticulum (ER), plasmalemma, and cytoplasm were isolated from RAW 264.7 macrophages in basal and activated states. Subsequent lipidomic analyses of major membrane lipid categories identified 229 individual/isobaric species, including 163 glycerophospholipids, 48 sphingolipids, 13 sterols, and 5 prenols. Major subcellular compartments exhibited substantially divergent glycerophospholipid profiles. Activation of macrophages by the Toll-like receptor 4-specific lipopolysaccharide Kdo2-lipid A caused significant remodeling of the subcellular lipidome. Some changes in lipid composition occurred in all compartments (e.g. increases in the levels of ceramides and the cholesterol precursors desmosterol and lanosterol). Other changes were manifest in specific organelles. For example, oxidized sterols increased and unsaturated cardiolipins decreased in mitochondria, whereas unsaturated ether-linked phosphatidylethanolamines decreased in the ER. We speculate that these changes may reflect mitochondrial oxidative stress and the release of arachidonic acid from the ER in response to cell activation. |
| Institute | LIPID MAPS |
| Department | Multiple |
| Laboratory | Multiple |
| Last Name | Fahy |
| First Name | Eoin |
| Address | 9500 Gilman, La Jolla, CA, 92093, USA |
| efahy@ucsd.edu | |
| Phone | 858-534-4076 |
| Submit Date | 2019-12-17 |
| Publications | Andreyev AY, Fahy E, Guan Z, Kelly S, Li X, McDonald JG, Milne S, Myers D, Park H, Ryan A, Thompson BM, Wang E, Zhao Y, Brown HA, Merrill AH, Raetz CR, Russell DW, Subramaniam S, Dennis EA. Subcellular organelle lipidomics in TLR-4-activated macrophages. J Lipid Res. 2010 Sep;51(9):2785-97. doi: 10.1194/jlr.M008748. Epub 2010 Jun 23. PMID: 20574076; PMCID: PMC2918461. |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-01-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002141 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase/Normal phase |
| Chromatography system | Several |
| Column | Several |
| MS Type | ESI |
| MS instrument type | Several |
| MS instrument name | Several |
| Ion Mode | UNSPECIFIED |
| Units | pmoles per mg protein |
MS:
| MS ID: | MS001993 |
| Analysis ID: | AN002141 |
| Instrument Name: | Several |
| Instrument Type: | Several |
| MS Type: | ESI |
| MS Comments: | See publication for details. doi: 10.1194/jlr.M008748 |
| Ion Mode: | UNSPECIFIED |
