Summary of Study ST001302
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000883. The data can be accessed directly via it's Project DOI: 10.21228/M8JM4K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001302 |
| Study Title | Metabolome Profiling of a Fast-growing Cyanobacterium Synechococcus elongatus PCC 11801 under Diurnal Cycle |
| Study Type | Measurement of relative metabolite pools under diurnal cycle using isotopic ratio method |
| Study Summary | Experiments were carried out by growing Synechococcus elongatus PCC 11801 cells in multicultivators designed to provide sinusoidal light. The period was 14:10 (light-dark). The samples for metabolomics analysis were collected in the second diurnal cycle at an interval of 6 hours starting from 25th hour (25, 31, 37 and 43 hours). The light intensity amplitude was 600 µmole photons.m-2. s-1. Additionally samples were also collected using cells grown under continuous light illumination of 600 µmole photons.m-2. s-1 to compare the difference in metabolite levels compared to that in the diurnal cycle. |
| Institute | Indian Institute of Technology Bombay |
| Department | Department of Chemical Engineering |
| Laboratory | Biosystems Engineering Lab |
| Last Name | Wangikar |
| First Name | Pramod P |
| Address | Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, Maharashtra, India - 400076 |
| wangikar@iitb.ac.in | |
| Phone | +91 (22) 2576 7232 (o) |
| Submit Date | 2020-01-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-10-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002168 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu 20AD |
| Column | Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um) |
| MS Type | ESI |
| MS instrument type | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE |
| Units | Area Ratio |
MS:
| MS ID: | MS002017 |
| Analysis ID: | AN002168 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| MS Comments: | The peak areas corresponding to the 12C and 13C monoisotopic peak for the metabolites of interest were quantified using MultiQuant 3.0.1 (SCIEX, Framingham, MA). The relative quantification of metabolites was done using isotopic ratio method by normalizing area under the peak for monoisotopic m/z of a particular metabolite by its respective highest possible isotopologue present in the internal standard giving area ratio. |
| Ion Mode: | NEGATIVE |
