Summary of Study ST001323
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000900. The data can be accessed directly via it's Project DOI: 10.21228/M8BX09 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001323 |
| Study Title | Effect of high-fat diet on serum lipidome in mice |
| Study Summary | We analyzed mouse serum samples from a mouse dietary intervention experiment. Briefly, C57BL/6 mice (n=44) were divided into 4 groups (n=11 per group) and fed High-fat diet (HFD), 1% deoxycholic acid (DCA) in drinking water, both, or left as control for 9 months. For quality control, 12 TQC samples and 2 blanks were also included in the analysis (total 58 samples and 6 groups). The two treatments were selected to demonstrate the ability of lipidomics to detect gross changes induced by HFD in the serum lipidome, as well as specific/minor changes induced by the secondary bile acid (DCA) through regulation of liver lipid metabolism. |
| Institute | QIMR Berghofer Medical Research Institute |
| Last Name | Mohamed |
| First Name | Ahmed |
| Address | 300 Herston Road |
| ahmed.mohamed@qimrberghofer.edu.au | |
| Phone | +61738453992 |
| Submit Date | 2020-03-02 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-03-20 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002199 | AN002200 | AN002201 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | HILIC | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um) | Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um) | Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ | Agilent 6490 QQQ |
| Ion Mode | UNSPECIFIED | POSITIVE | UNSPECIFIED |
| Units | intensity | intensity | intensity |
MS:
| MS ID: | MS002045 |
| Analysis ID: | AN002199 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Targeted lipidomics were performed on an Agilent Technologies 1290 Infinity UHPLC system with an Agilent HILIC Plus RRHD 2.1×100 mm 1.8-micron column, coupled online to an Agilent 6490A Triple Quadrupole mass spectrometer with iFunnel and Agilent Jet Stream (AJS) electrospray ionization (ESI) source, operated in dynamic MRM mode. The source nitrogen gas temperature was set to 250°C at a flow of 15 L/min and a sheath gas temperature of 400°C at a flow of 12 L/min. The capillary voltage was set to 4000 V for positive mode and 5000 V for negative mode and the nebulizer operated at 30 psi. Ion funnel high and low pressure in positive and negative mode were 150 and 120. Check tunes were performed in wide, unit and enhanced modes prior to each experiment to confirm the performance of the mass spectrometer. The quadrupole was tuned to reference masses 118.09, 322.05, 622.03, 922.01 and 1221.99 in positive ionization mode; 112.99, 302.00, 601.98, 1033.99 and 1333.97 in negative ionization mode. |
| Ion Mode: | UNSPECIFIED |
| MS ID: | MS002046 |
| Analysis ID: | AN002200 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Targeted lipidomics were performed on an Agilent Technologies 1290 Infinity UHPLC system with an Agilent HILIC Plus RRHD 2.1×100 mm 1.8-micron column, coupled online to an Agilent 6490A Triple Quadrupole mass spectrometer with iFunnel and Agilent Jet Stream (AJS) electrospray ionization (ESI) source, operated in dynamic MRM mode. The source nitrogen gas temperature was set to 250°C at a flow of 15 L/min and a sheath gas temperature of 400°C at a flow of 12 L/min. The capillary voltage was set to 4000 V for positive mode and 5000 V for negative mode and the nebulizer operated at 30 psi. Ion funnel high and low pressure in positive and negative mode were 150 and 120. Check tunes were performed in wide, unit and enhanced modes prior to each experiment to confirm the performance of the mass spectrometer. The quadrupole was tuned to reference masses 118.09, 322.05, 622.03, 922.01 and 1221.99 in positive ionization mode; 112.99, 302.00, 601.98, 1033.99 and 1333.97 in negative ionization mode. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002047 |
| Analysis ID: | AN002201 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Targeted lipidomics were performed on an Agilent Technologies 1290 Infinity UHPLC system with an Agilent HILIC Plus RRHD 2.1×100 mm 1.8-micron column, coupled online to an Agilent 6490A Triple Quadrupole mass spectrometer with iFunnel and Agilent Jet Stream (AJS) electrospray ionization (ESI) source, operated in dynamic MRM mode. The source nitrogen gas temperature was set to 250°C at a flow of 15 L/min and a sheath gas temperature of 400°C at a flow of 12 L/min. The capillary voltage was set to 4000 V for positive mode and 5000 V for negative mode and the nebulizer operated at 30 psi. Ion funnel high and low pressure in positive and negative mode were 150 and 120. Check tunes were performed in wide, unit and enhanced modes prior to each experiment to confirm the performance of the mass spectrometer. The quadrupole was tuned to reference masses 118.09, 322.05, 622.03, 922.01 and 1221.99 in positive ionization mode; 112.99, 302.00, 601.98, 1033.99 and 1333.97 in negative ionization mode. |
| Ion Mode: | UNSPECIFIED |
