Summary of Study ST001330
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000909. The data can be accessed directly via it's Project DOI: 10.21228/M8668V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001330 |
| Study Title | Multi-omics of OsGF14b-mediated innate immunity against panicle blast in rice |
| Study Summary | In the present study, we used a multi-omics approach to decipher the molecular mechanisms of OsGF14b in governing panicle resistance to Magnaporthe oryzae.Results revealed OsGF14b mediated panicle blast resistance was involved in the activation of auxin and JA signaling pathways, resulting in reprogramming of the phenylpropanoid and diterpenoid pathway. |
| Institute | Agro-biological Gene Research Center , Guangdong Academy of Agricultural Sciences |
| Last Name | Yan |
| First Name | Shijuan |
| Address | No. 20 Jinying Road, Tianhe District, Guangzhou City, Guangdong Province, 510640, China. |
| shijuan@agrogene.ac.cn | |
| Phone | +86-020-38213643 |
| Submit Date | 2020-03-13 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2020-12-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002216 | AN002217 | AN002218 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 7890A | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Agilent DB35-MS (30m x 0.25mm,0.25um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | EI | ESI | ESI |
| MS instrument type | Single quadrupole | Orbitrap | Orbitrap |
| MS instrument name | Agilent 5975 | Thermo Fusion Tribrid Orbitrap | Thermo Fusion Tribrid Orbitrap |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
| Units | Relative content | Relative content | Relative content |
MS:
| MS ID: | MS002062 |
| Analysis ID: | AN002216 |
| Instrument Name: | Agilent 5975 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | The transfer line temperature was set to 300°C, and the ion source temperature was set to 230°C. The mass range analyzed was from m/z 85 to 700. The Agilent MassHunter Qualitative Analysis (version B06.00) software and the Agilent MassHunter Quantitative Analysis (version B.07.01) were jointly used for GC-MS data analyses. NIST library and in-house database established using authentic standards were used together for metabolite identification. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002063 |
| Analysis ID: | AN002217 |
| Instrument Name: | Thermo Fusion Tribrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The spray voltage was set to 3500 V in the positive-ion modes, with the following ion-source properties: ion source type, H-ESI; Sheath gas, 45 Arb; Aux gas, 10 Arb; sweep gas, 0 Arbs; Ion transfer tube temperature, 320 °C; Vaporizer temperature, 350 °C. Full scan data was acquired with a scan range of m/z 100-1000, which was acquired with orbitrap resolution of 120000. The automatic gain control (AGC) was set at 2E5 and the maximum injection time was set to 100 ms. RF lens was set to 60%, and the micro scans was 1. data type, profile. All FTMS2 data were acquired using the following conditions: isolation mode, quadrupole; isolation window, 1.6 m/z; detector type, Orbitrap; scan range, auto; AGC target, 5.0e4; maximum injection time, 35 ms; microscans, 1; orbitrap resolution, 15000; first mass, 100 m/z; data type, profile. Both HCD and CID were used for FTMS2 as the activation type. The HCD collision energy was set to 30%, 40%, and 50%, respectively, which ± HCD collision energy was set 10%. The CID collision energy was set to 30% and 40%, and the activation Q was set to 0.25. The Xcalibur v4.1 software (Thermo Fisher Scientific, USA) were used for HPLC-MS control. Compound Discovery (Thermo Fisher Scientific, San Jose, CA, USA) and Trace Finder 3.3 (Thermo Fisher Scientific, San Jose, CA, USA) were used for the LC-MS-based secondary metabolome data analysis, combining qualitative and quantitative analysis. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002064 |
| Analysis ID: | AN002218 |
| Instrument Name: | Thermo Fusion Tribrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The spray voltage was set to -3000 V in the negative-ion modes, with the following ion-source properties: ion source type, H-ESI; Sheath gas, 45 Arb; Aux gas, 10 Arb; sweep gas, 0 Arbs; Ion transfer tube temperature, 320 °C; Vaporizer temperature, 350 °C. Full scan data was acquired with a scan range of m/z 100-1000, which was acquired with orbitrap resolution of 120000. The automatic gain control (AGC) was set at 2E5 and the maximum injection time was set to 100 ms. RF lens was set to 60%, and the micro scans was 1. data type, profile. All FTMS2 data were acquired using the following conditions: isolation mode, quadrupole; isolation window, 1.6 m/z; detector type, Orbitrap; scan range, auto; AGC target, 5.0e4; maximum injection time, 35 ms; microscans, 1; orbitrap resolution, 15000; first mass, 100 m/z; data type, profile. Both HCD and CID were used for FTMS2 as the activation type. The HCD collision energy was set to 30%, 40%, and 50%, respectively, which ± HCD collision energy was set 10%. The CID collision energy was set to 30% and 40%, and the activation Q was set to 0.25. The Xcalibur v4.1 software (Thermo Fisher Scientific, USA) were used for HPLC-MS control. Compound Discovery (Thermo Fisher Scientific, San Jose, CA, USA) and Trace Finder 3.3 (Thermo Fisher Scientific, San Jose, CA, USA) were used for the LC-MS-based secondary metabolome data analysis, combining qualitative and quantitative analysis. |
| Ion Mode: | NEGATIVE |
