Summary of Study ST001361
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000931. The data can be accessed directly via it's Project DOI: 10.21228/M8BT4S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001361 |
Study Title | Serum tryptophan metabolomics in CKD |
Study Type | MS quantitative analysis |
Study Summary | Serum was processed using a targeted metabolomics platform for quantifying tryptophan metabolites as a number of these metabolites are well establish uremic toxins. |
Institute | University of Florida |
Last Name | Ryan |
First Name | Terence |
Address | 1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA |
ryant@ufl.edu | |
Phone | 352-294-1700 |
Submit Date | 2020-04-02 |
Num Groups | 2 |
Total Subjects | 16 |
Num Males | 8 |
Num Females | 8 |
Raw Data Available | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2020-12-31 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002265 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex |
Column | ACE 5 C18-300 (100 x 2.1mm) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | ng/ml |
MS:
MS ID: | MS002109 |
Analysis ID: | AN002265 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Tryptophan, serotonin, kynurenine, kynurenic acid, xanthurenic acid and anthranilic acid were quantified in the positive ionization while indoxyl sulfate, p-cresol sulfate and 3-indole-acetate were analyzed in negative ionization. Separation was achieved on an ACE 18-PFP 100 x 2.1 mm, 2 µm column using a gradient with mobile phase A as 0.1% formic acid in water and mobile phase B as acetonitrile. The flow rate was 350 µL/min with a column temperature of 25°C and injection volume of 2 µL. Run time was 20.5 min. A 9-point calibration curve and QC samples were prepared for targeted quantitation of tryptophan, serotonin, kynurenine, kynurenic acid, xanthurenic acid, anthranilic acid, indoxyl sulfate, p-cresol sulfate and 3-indole-acetate. 20 µL of each calibrator and QCs were supplemented with 5 µl indoxyl sulfate. Peak areas of each analyte and corresponding internal standard in the calibrator, QCs and samples were integrated using Xcalibur 4.0. A calibration curve was generated by plotting nominal concentration of the analyte in the calibrators versus peak area ratio of analyte |
Ion Mode: | POSITIVE |