Summary of Study ST001361

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000931. The data can be accessed directly via it's Project DOI: 10.21228/M8BT4S This work is supported by NIH grant, U2C- DK119886.

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Study IDST001361
Study TitleSerum tryptophan metabolomics in CKD
Study TypeMS quantitative analysis
Study SummarySerum was processed using a targeted metabolomics platform for quantifying tryptophan metabolites as a number of these metabolites are well establish uremic toxins.
Institute
University of Florida
Last NameRyan
First NameTerence
Address1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA
Emailryant@ufl.edu
Phone352-294-1700
Submit Date2020-04-02
Num Groups2
Total Subjects16
Num Males8
Num Females8
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2020-12-31
Release Version1
Terence Ryan Terence Ryan
https://dx.doi.org/10.21228/M8BT4S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002265
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex
Column ACE 5 C18-300 (100 x 2.1mm)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode POSITIVE
Units ng/ml

MS:

MS ID:MS002109
Analysis ID:AN002265
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Tryptophan, serotonin, kynurenine, kynurenic acid, xanthurenic acid and anthranilic acid were quantified in the positive ionization while indoxyl sulfate, p-cresol sulfate and 3-indole-acetate were analyzed in negative ionization. Separation was achieved on an ACE 18-PFP 100 x 2.1 mm, 2 µm column using a gradient with mobile phase A as 0.1% formic acid in water and mobile phase B as acetonitrile. The flow rate was 350 µL/min with a column temperature of 25°C and injection volume of 2 µL. Run time was 20.5 min. A 9-point calibration curve and QC samples were prepared for targeted quantitation of tryptophan, serotonin, kynurenine, kynurenic acid, xanthurenic acid, anthranilic acid, indoxyl sulfate, p-cresol sulfate and 3-indole-acetate. 20 µL of each calibrator and QCs were supplemented with 5 µl indoxyl sulfate. Peak areas of each analyte and corresponding internal standard in the calibrator, QCs and samples were integrated using Xcalibur 4.0. A calibration curve was generated by plotting nominal concentration of the analyte in the calibrators versus peak area ratio of analyte
Ion Mode:POSITIVE
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