Summary of Study ST001364
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000928. The data can be accessed directly via it's Project DOI: 10.21228/M8R094 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001364 |
| Study Title | Core Functional Nodes and Sex-Specific Pathways in Human Ischemic and Dilated Cardiomyopathy |
| Study Summary | Restricted access to human left ventricular myocardium is a significant limitation in the study of heart failure (HF). Here, we utilise a large human heart biobank of carefully procured, cryopreserved left ventricular myocardium to obtain direct molecular insights into ischaemic (ICM) and dilated cardiomyopathy (DCM), the most common causes of HF worldwide1. We performed unbiased, deep proteomic and metabolomic analyses of 51 left ventricular (LV) samples from 44 cryopreserved human ICM and DCM hearts, including age-matched, histopathologically normal, donor controls of both genders for comparison. For the first time, we report perturbed thyroid hormone signalling pathways in the myocardium of both types of HF, and unveil the interaction of gender with HF, including increased nitric oxide-related arginine metabolism in male hearts, and many gender-specific mitochondrial and X chromosome-linked protein and metabolite changes. We provide all raw data, in addition to an interactive online application, as a publicly-available resource. |
| Institute | University of Sydney |
| Last Name | John |
| First Name | O'Sullivan |
| Address | Johns Hopkins Dr, Camperdown NSW 2006, Australia |
| john.osullivan@sydney.edu.au | |
| Phone | +447731801851 |
| Submit Date | 2019-12-07 |
| Raw Data Available | Yes |
| Analysis Type Detail | API-MS |
| Release Date | 2020-04-30 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002270 | AN002271 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1260 | Agilent 1260 |
| Column | Waters Atlantis HILIC (100 x 2.1mm,3um,100Å) | Waters XBridge Amide (100 x 4.6mm,3.5um) |
| MS Type | API | API |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | intensity (arbitrary unit) | intensity (arbitrary unit) |
MS:
| MS ID: | MS002114 |
| Analysis ID: | AN002270 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | API |
| MS Comments: | Interface: Turboionspray, positive ionization mode Scan Mode: Scheduled multiple reaction monitoring (MRM) sMRM Window: 30 sec Target Scan Time: 1.0 sec Source Temp: 450°C Ion Source position: Vertical: 1, Horizontal: 5 |
| Ion Mode: | POSITIVE |
| MS ID: | MS002115 |
| Analysis ID: | AN002271 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | API |
| MS Comments: | Interface: Turboionspray, negative ionization mode Scan Mode: Scheduled multiple reaction monitoring (MRM) sMRM Window: 30 sec Target Scan Time: 1.0 sec Source Temp: 450°C Ion Source position: Vertical: 1, Horizontal: 5 |
| Ion Mode: | NEGATIVE |
