Summary of Study ST001384

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000948. The data can be accessed directly via it's Project DOI: 10.21228/M8540T This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001384
Study TitlePlasmodium falciparum increased time in circulation underlies persistent asymptomatic infection in the dry season
Study SummaryThe dry season is a major challenge for Plasmodium falciparum parasites in many malaria endemic regions, where water availability limits mosquitoes to only part of the year. How P. falciparum bridges two transmission seasons months apart, without being cleared by the host or compromising host survival is poorly understood. Here we show that low levels of P. falciparum parasites persist in the blood of asymptomatic Malian individuals during the 5- to 6-month dry season, rarely causing symptoms and minimally affecting the host immune response. Parasites isolated during the dry season are transcriptionally distinct from those of subjects with febrile malaria in the transmission season, reflecting longer circulation within each replicative cycle, of parasitized erythrocytes without adhering to the vascular endothelium. Low parasite levels during the dry season are not due to impaired replication, but rather increased splenic clearance of longer-circulating infected erythrocytes. We propose that P. falciparum virulence in areas of seasonal malaria transmission is regulated so that the parasite decreases its endothelial binding capacity, allowing increased splenic clearance and enabling several months of subclinical parasite persistence.
Institute
Pennsylvania State University
Last NameLlinas
First NameManuel
AddressW126 Millennium Science Complex, University Park, PENNSYLVANIA, 16802, USA
Emailmanuel@psu.edu
Phone(814) 867-3527
Submit Date2020-05-25
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2020-08-20
Release Version1
Manuel Llinas Manuel Llinas
https://dx.doi.org/10.21228/M8540T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002308 AN002309 AN002310 AN002311
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase Reversed phase
Chromatography system Shimadzu Prominence 20 UFLCXR Thermo Dionex Ultimate 3000 Shimadzu Prominence 20 UFLCXR Shimadzu Prominence 20 UFLCXR
Column Waters BEH C18 (100mm x 2.1mm; 1.7um) Waters XSelect HSS T3 (100 x 2.1mm,2.5um) Waters Acquity CSH C18 (100 x 2.1mm,1.7um) Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI ESI ESI
MS instrument type QTOF Orbitrap QTOF QTOF
MS instrument name ABI Sciex 5600 TripleTOF Thermo Exactive Plus Orbitrap ABI Sciex 5600 TripleTOF ABI Sciex 5600 TripleTOF
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Area Area Area

MS:

MS ID:MS002151
Analysis ID:AN002308
Instrument Name:ABI Sciex 5600 TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The capillary voltage was set at 4.5 kV in negative ion mode, with a declustering potential of 80V. The mass spectrometer was operated in IDA (Information Dependent Acquisition) mode with a 100 ms survey scan from 100 to 1200 m/z, and up to 20 MS/MS product ion scans (100 ms) per duty cycle using a collision energy of 50V with a 20V spread. Data processing was performed using MS-DIAL and annotated using the built-in public library. Blank subtraction and analysis was performed in excel.
Ion Mode:POSITIVE
  
MS ID:MS002152
Analysis ID:AN002309
Instrument Name:Thermo Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Aquired in MS1 mode only. Data processing and annotation performed using MAVEN and an in-house targeted list. Blank subtraction and analysis was performed in excel.
Ion Mode:NEGATIVE
  
MS ID:MS002153
Analysis ID:AN002310
Instrument Name:ABI Sciex 5600 TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:IDA MS2 method. Ammonium formate and formic acid were added to the positive ESI solvents. Data processing performed using MS-DIAL and annotated using the in silico library. Blank subtraction and analysis was performed in excel.
Ion Mode:POSITIVE
  
MS ID:MS002154
Analysis ID:AN002311
Instrument Name:ABI Sciex 5600 TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:IDA MS2 method. Ammonium acetate was used for negative ESI solvents. Data processing performed using MS-DIAL and annotated using the in silico library. Blank subtraction and analysis was performed in excel.
Ion Mode:NEGATIVE
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