Summary of Study ST001393

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000956. The data can be accessed directly via it's Project DOI: 10.21228/M84386 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001393
Study TitleSea-ice diatom compatible solute shifts
Study TypeCompatible solutes were quantified in sea-ice diatoms
Study SummarySea-ice algae provide an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures of the Antarctic sea-ice diatom, Nitzschia lecointei, displayed large differences in their metabolomes when grown in a matrix of conditions that included temperatures of –1 and 4°C, and salinities of 32 and 41, despite relatively small changes in growth rate. Temperature exerted a greater effect than salinity on cellular metabolite pool sizes, though the N- or S-containing compatible solutes, 2,3-dihydroxypropane-1-sulfonate (DHPS), glycine betaine (GBT), dimethylsulfoniopropionate (DMSP), and proline responded strongly to both temperature and salinity, suggesting complexity in their control. We saw the largest (> 4 fold) response to salinity for proline. DHPS, a rarely studied but potential compatible solute, reached the highest intracellular compatible solute concentrations of ~ 85 mM. When comparing the culture findings to natural Arctic sea-ice diatom communities, we found extensive overlap in metabolite profiles, highlighting the relevance of culture-based studies to probe environmental questions. Large changes in sea-ice diatom metabolomes and compatible solutes over a seasonal cycle could be significant components of biogeochemical cycling within sea ice.
Institute
University of Washington
DepartmentSchool of Oceanography
LaboratoryIngalls Lab
Last NameDawson
First NameHannah
Address1501 NE Boat Street, Marine Science Building, Room G, Seattle, WA 98195
Emailhmdawson@uw.edu
Phone2062216750
Submit Date2020-03-24
PublicationsDawson et al., Elementa
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2020-09-29
Release Version1
Hannah Dawson Hannah Dawson
https://dx.doi.org/10.21228/M84386
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002323 AN002324 AN002325 AN002326 AN002327 AN002328 AN002329 AN002330 AN002331
Analysis type MS MS MS MS MS MS MS MS MS
Chromatography type HILIC HILIC HILIC HILIC HILIC HILIC HILIC HILIC Reversed phase
Chromatography system Waters Acquity I-Class Waters Acquity I-Class Waters Acquity I-Class Waters Acquity I-Class Waters Acquity I-Class Waters Acquity I-Class Waters Acquity I-Class Waters Acquity I-Class Waters Acquity I-Class
Column SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um) Waters Acquity UPLC HSS Cyano (100 x 2.1mm,1.8um)
MS Type ESI ESI ESI ESI ESI ESI ESI ESI ESI
MS instrument type Triple quadrupole Triple quadrupole Triple quadrupole Triple quadrupole Orbitrap Orbitrap Orbitrap Orbitrap Triple quadrupole
MS instrument name Waters Xevo-TQ-S Waters Xevo-TQ-S Waters Xevo-TQ-S Waters Xevo-TQ-S Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap Waters Xevo-TQ-S
Ion Mode POSITIVE POSITIVE NEGATIVE NEGATIVE POSITIVE POSITIVE NEGATIVE NEGATIVE POSITIVE
Units Normalized Peak Area Per L Seawater Normalized Peak Area Per RFU Normalized Peak Area Per L Seawater Normalized Peak Area Per RFU Normalized Peak Area Per L Seawater Normalized Peak Area Per RFU Normalized Peak Area Per L Seawater Normalized Peak Area Per RFU Normalized Peak Area Per RFU

MS:

MS ID:MS002165
Analysis ID:AN002323
Instrument Name:Waters Xevo-TQ-S
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:See protocol, data from culture samples
Ion Mode:POSITIVE
  
MS ID:MS002166
Analysis ID:AN002324
Instrument Name:Waters Xevo-TQ-S
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:See protocol, data from field samples
Ion Mode:POSITIVE
  
MS ID:MS002167
Analysis ID:AN002325
Instrument Name:Waters Xevo-TQ-S
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:See protocol, data from culture samples
Ion Mode:NEGATIVE
  
MS ID:MS002168
Analysis ID:AN002326
Instrument Name:Waters Xevo-TQ-S
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:See protocol, data from field samples
Ion Mode:NEGATIVE
  
MS ID:MS002169
Analysis ID:AN002327
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:See protocol, data from culture samples
Ion Mode:POSITIVE
  
MS ID:MS002170
Analysis ID:AN002328
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:See protocol, data from field samples
Ion Mode:POSITIVE
  
MS ID:MS002171
Analysis ID:AN002329
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:See protocol, data from culture samples
Ion Mode:NEGATIVE
  
MS ID:MS002172
Analysis ID:AN002330
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:See protocol, data from field samples
Ion Mode:NEGATIVE
  
MS ID:MS002173
Analysis ID:AN002331
Instrument Name:Waters Xevo-TQ-S
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:See protocol, data from culture samples
Ion Mode:POSITIVE
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