Summary of Study ST001687

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001085. The data can be accessed directly via it's Project DOI: 10.21228/M8FT4Q This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001687
Study TitleNon-transformed cells respond to fat by inducing glucose metabolism
Study SummaryC57BL/6N mice were obtained from the KU Leuven animal laboratory. 2-week old mice were injected i.p. diethylnitrosamine (DEN, 25 mg/Kg) in phosphate buffered saline (3.17 mg/ml).s At 6-weeks of age, mice were randomized into two groups: control diet (CD, E15742-33 ssniff Spezialdiäten GmbH) or high fat diet (HFD, S8655-E220 sniff Spezialdiäten GmbH). At endpoint, mice were sacrificed by injecting approximately 50 µl of a 60 mg/ml Nembutal solution (Vetoquinol). Tissues were immediately excised, washed in ice-cold saline, placed into pre-labelled bags and frozen using a liquid nitrogen-cooled biosqueezer. The bags were then placed in liquid nitrogen until all collections were finished and finally stored at -80°C until further processing. Where necessary tumors were rapidly separated from normal tissue prior to freezing with tumor and normal tissue being stored separately.
Institute
VIB-KU Leuven Center for Cancer Biology
DepartmentDepartment of Oncology
LaboratoryLaboratory of Cellular Metabolism and Metabolic Regulation
Last NameFendt
First NameSarah-Maria
AddressON IV Herestraat 49 - box 912 3000 Leuven
Emailsarah-maria.fendt@kuleuven.be
Phone+32 16 37 32 61
Submit Date2021-02-04
Num Groups4
Total Subjects16
Num Males16
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-02-27
Release Version1
Sarah-Maria Fendt Sarah-Maria Fendt
https://dx.doi.org/10.21228/M8FT4Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002754 AN002755
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Shimadzu Nexera X2 Shimadzu Nexera X2
Column Waters Xbridge Amide (150 x 4.6mm,3.5um) Waters Xbridge Amide (150 x 4.6mm,3.5um)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 6500+ QTRAP ABI Sciex 6500+ QTRAP
Ion Mode POSITIVE NEGATIVE
Units nmol lipid/mg DNA nmol lipid/mg DNA

MS:

MS ID:MS002551
Analysis ID:AN002754
Instrument Name:ABI Sciex 6500+ QTRAP
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS002552
Analysis ID:AN002755
Instrument Name:ABI Sciex 6500+ QTRAP
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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