Summary of Study ST001752

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001123. The data can be accessed directly via it's Project DOI: 10.21228/M8JD7N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001752
Study TitleDual RNA regulator VcdRP in V. cholerae modulates central metabolism
Study SummaryBacterial small RNAs (sRNAs) are well-known to modulate gene expression by base-pairing with trans-coded transcripts and are typically considered to be non-coding. However, several sRNAs have been reported to also contain an open reading frame and thus are considered dual-function regulators. We discovered a dual-function regulator from Vibrio cholerae, called VcdRP, harboring a 29 amino acid protein (VcdP), as well as a base-pairing sequence. In this study, we measured the metabolite abundance of glycolytic and citric acid cycle intermediates using LC-MS.
Institute
Helmholtz Centre for Environmental Research
DepartmentMolecular Systems Biology
LaboratoryFunctional Metabolomics
Last NameEngelmann
First NameBeatrice
AddressPermoserstraße 15, Leipzig, Saxony, 03418, Germany
Emailbeatrice.engelmann@ufz.de
Phone00493412351099
Submit Date2021-04-14
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2021-05-01
Release Version1
Beatrice Engelmann Beatrice Engelmann
https://dx.doi.org/10.21228/M8JD7N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002855
Analysis type MS
Chromatography type Ion pair
Chromatography system Agilent 1290 Infinity II
Column Waters XSelect HSS T3 XP (150 x 2.1mm,2.5um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name AB Sciex 6500+ QTRAP
Ion Mode NEGATIVE
Units relative abundance

MS:

MS ID:MS002648
Analysis ID:AN002855
Instrument Name:AB Sciex 6500+ QTRAP
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:MS acquisition as well as data processing software was Analyst vs 1.7.0
Ion Mode:NEGATIVE
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