Summary of Study ST001973

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001253. The data can be accessed directly via it's Project DOI: 10.21228/M8R98V This work is supported by NIH grant, U2C- DK119886.

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Study IDST001973
Study TitleAnalytical methodology for a metabolome atlas of goat’s plasma, milk and feces using 1H-NMR and UHPLC-HRMS:MS/feces
Study SummaryMetabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factors that can also alter animal integrity and welfare. Some studies have already investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (Nuclear Magnetic Resonance or Mass Spectrometry). Only few studies with multi-platform approaches are also used with a single or a multi type of sample, but they mainly consider dairy cows metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabolic atlas of goat plasma, milk, and feces, based on healthy animals. Our study describes a Standard Operating Procedure for three goat matrices: blood plasma, milk, and feces using multiple platforms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample preparation procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.
Institute
INSERM
DepartmentINSERM
Last NameMartias
First NameCecile
Address10 Boulevard tonnelé, Indre et Loire, 37000, France
Emailcecile.martias@univ-tours.fr
Phone0247366351
Submit Date2021-09-26
Analysis Type DetailLC-MS
Release Date2022-01-02
Release Version1
Cecile Martias Cecile Martias
https://dx.doi.org/10.21228/M8R98V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003218 AN003219 AN003220 AN003221
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase HILIC HILIC
Chromatography system Phenomenex Phenomenex Waters Acquity Waters Acquity
Column Phenomenex Kinetex XB-C18 (150 x 2.1mm,1.7um,100A) Phenomenex Kinetex XB-C18 (150 x 2.1mm,1.7um,100A) Waters Cortecs unbonded silica (150 x 2.1mm,1.6um,100 A ) Waters Cortecs unbonded silica (150 x 2.1mm,1.6um,100 A )
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units SI SI SI SI

MS:

MS ID:MS002992
Analysis ID:AN003218
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS002993
Analysis ID:AN003219
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
  
MS ID:MS002994
Analysis ID:AN003220
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS002995
Analysis ID:AN003221
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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