Summary of Study ST002063

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001306. The data can be accessed directly via it's Project DOI: 10.21228/M8WT4R This work is supported by NIH grant, U2C- DK119886.


Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002063
Study TitleIntravenous lipopolysaccharide infusion and the bovine metabolome
Study TypeMS plasma lipidomics and metabolomics
Study SummaryThe effects of lipopolysaccharides (i.e., endotoxin; LPS) on metabolism are poorly defined in lactating dairy cattle experiencing hyperlipidemia. Our objective was to explore the effects of acute intravenous LPS administration on metabolism in late-lactation Holstein cows experiencing hyperlipidemia. Ten non-pregnant lactating Holstein cows (273 ± 35 d in milk) were administered a single bolus of saline (3 mL of saline; n = 5) or LPS (0.375 μg of LPS/kg of body weight; n = 5). Simultaneously, cows were intravenously infused a triglyceride emulsion and fasted for 16 h to induce hyperlipidemia in an attempt to model the periparturient period. Blood was sampled at routine intervals. Changes in circulating total fatty acid concentrations and inflammatory parameters were measured. Plasma samples were analyzed using untargeted lipidomics and metabolomics. Endotoxin increased circulating serum amyloid A, LPS-binding protein, and cortisol concentrations. Endotoxin administration decreased plasma lysophosphatidylcholine (LPC) concentrations and increased select plasma ceramide concentrations. These outcomes suggest modulation of the immune response and insulin action. Lipopolysaccharide decreased the ratio of phosphatidylcholine to phosphatidylethanomanine, which potentially indicate a decrease in the hepatic activation of phosphatidylethanolamine N-methyltransferase and triglyceride export. Endotoxin administration also increased plasma concentrations of pyruvic and lactic acids, and decreased plasma citric acid concentrations, which implicate the upregulation of glycolysis and downregulation of the citric acid cycle (i.e., the Warburg effect), potentially in leukocytes. Acute intravenous LPS administration decreased circulating LPC concentrations, modified ceramide and glycerophospholipid concentrations, and influenced intermediary metabolism in dairy cows experiencing hyperlipidemia.
Cornell University
DepartmentAnimal Science
LaboratoryMcFadden lab
Last NameJavaid
First NameAwais
Address400 Warren Rd, Ithaca, New York, 14850, USA
Submit Date2022-01-09
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-06-30
Release Version1
Awais Javaid Awais Javaid application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN003362
Analysis type MS
Chromatography type Reversed phase
Chromatography system Vanquish UHPLC system
Column Accucore C30 (150 x 2.1mm,2.6um)
MS instrument type Single quadrupole
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Units Normalized ion intensity


MS ID:MS003131
Analysis ID:AN003362
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Single quadrupole
MS Comments:Please refer to publication Wang et al 2022.