Summary of Study ST002159
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001372. The data can be accessed directly via it's Project DOI: 10.21228/M8CH8H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002159 |
| Study Title | Chemotaxonomic patterns in intracellular metabolites of marine microbial plankton |
| Study Summary | Targeted and untargeted metabolomes were generated for a variety of marine microbial taxa including eukaryotic phytoplankton, cyanobacteria, archaea, and heterotrophic bacteria. Microbial metabolism generates small organic molecules that reflect both the biochemical and physiological diversity as well as the taxonomic specificity of biological processes. These small molecules serve as the conduit for taxon-specific signaling and exchange. We used liquid chromatography-mass spectrometry (LC-MS)-based metabolomics to taxonomically categorize metabolites that include small molecules in central and secondary metabolism across 42 taxa representing numerically dominant and metabolically important lineages of microbial autotrophs and heterotrophs. |
| Institute | University of Washington |
| Department | Oceanography |
| Laboratory | Ingalls |
| Last Name | Durham |
| First Name | Bryndan |
| Address | 2033 Mowry Rd., CGRCRm 404 |
| b.durham@ufl.edu | |
| Phone | 3522946312 |
| Submit Date | 2021-12-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-05-16 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003535 | AN003536 | AN003537 | AN003538 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | HILIC | Reversed phase |
| Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class |
| Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | Waters Acquity UPLC HSS Cyano (100 x 2.1 mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Waters Xevo-TQ-S | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | UNSPECIFIED | POSITIVE | NEGATIVE | POSITIVE |
| Units | presence (1), absence (0), or background level (NA) | presence (1), absence (0), or background level (NA) | presence (1), absence (0), or background level (NA) | presence (1), absence (0), or background level (NA) |
MS:
| MS ID: | MS003293 |
| Analysis ID: | AN003535 |
| Instrument Name: | Waters Xevo-TQ-S |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | MS acquisition according to attached protocol. |
| Ion Mode: | UNSPECIFIED |
| Analysis Protocol File: | Ingalls_Metabolomics_MS_Parameters_2015.txt |
| MS ID: | MS003294 |
| Analysis ID: | AN003536 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS acquisition according to attached protocol. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Ingalls_Metabolomics_MS_Parameters_2015.txt |
| MS ID: | MS003295 |
| Analysis ID: | AN003537 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS acquisition according to attached protocol. |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Ingalls_Metabolomics_MS_Parameters_2015.txt |
| MS ID: | MS003296 |
| Analysis ID: | AN003538 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS acquisition according to attached protocol. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Ingalls_Metabolomics_MS_Parameters_2015.txt |
