Summary of Study ST002197
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001400. The data can be accessed directly via it's Project DOI: 10.21228/M8RM5T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002197 |
| Study Title | Metabolic signature of C-protein induced myositis mouse model |
| Study Summary | Objective of this study was to find Metabolic signature of C-protein induced myositis mouse model. For metabolite quantification, we used the tissue samples of C-protein induced myositis mouse model and control. Biocrates p180 kit enables to quantify 188 metabolites including amino acids, biogenic amines, acylcarnitine, glycerophospholipid and sugar. Significant metabolites were selected by t-test with false discovery rate p-value less than 0.05. In mouse tissue sample, a total of 68 metabolites significantly changed in CIM mice. We did pathway analysis to find altered pathway in muscle tissue. Metabolites from polyamine and beta-alanine pathway were altered. |
| Institute | Seoul National University |
| Last Name | Kang |
| First Name | Jihyun |
| Address | 502ho Intergrative Biomedical Education Research Building, 101 Deahakro, Jongro-gu, Seoul, 03080, Korea, South Korea |
| jikang@snu.ac.kr | |
| Phone | +821071014069 |
| Submit Date | 2022-06-15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-07-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003596 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | AB SCIEX QTRAP 5500 |
| Column | Waters ACQUITY UPLC BEH C18 (75 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE |
| Units | ng/mL |
MS:
| MS ID: | MS003351 |
| Analysis ID: | AN003596 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | For LC part: Mobile phase A: 1000 mL water + 2 mL formic acid (FA) Mobile phase B: 500 mL ACN + 1 mL FA Wash solvent 1: 500 mL ACN + 200 mL MeOH + 150 mL iPrOH + 150 mL water + 2.5 mL FA Wash solvent 2: water Install column and precolumn (if required). Wash the column at 95% B and condition to 100% For FIA part: Deactivate “Use flat files for scan data” in the Analyst software FIA mobile phase: FIA Mobile Phase Additive (1x glass ampule) + 290 mL MeOH Wash solvent 1: 500 mL ACN + 200 mL MeOH + 150 mL iPrOH + 150 mL water + 2.5 mL FA Wash solvent 2 (if possible): 333 mL MeOH + 333 mL iPrOH + 333 mL water For data processing: Adjust the retention times in the quantitation method and if necessary some integration parameters. Process the LC-MS data using the Quantitation Wizard. Save the results. Import the results export (TXT file) into MetIDQTM Parse the FIA-MS WIFF files directly into MetIDQTM. Evaluate results, perform statistics (e.g. using MetaboINDICATOR™, StatPack, MetaboAnalyst). |
| Ion Mode: | POSITIVE |
