Summary of Study ST002236
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001426. The data can be accessed directly via it's Project DOI: 10.21228/M8D42R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002236 |
| Study Title | The impact of IgE in gut and serum metabolomes in a murine experimental model of allergic enteritis |
| Study Type | Case-control study |
| Study Summary | The pathological mechanism of the gastrointestinal forms of food allergies is less understood in comparison to other clinical phenotypes, such as asthma, and anaphylaxis, partly due to difficulty in the access to intestinal tissues and because of a highly complex interplay between microbiota and intestinal mucosa. Importantly, a high level of IgE is a poor prognostic factor in gastrointestinal allergies. This study aimed to investigate how IgE influences the development of intestinal inflammation and the metabolome in allergic enteritis (AE), using IgE knock-in (IgEki) mice expressing high levels of IgE. Ovalbumin-sensitized and egg-white diet fed (OVA/EW) BALB/c WT mice developed moderate AE, whereas OVA/EW IgEki mice induced more aggravated intestinal inflammation with enhanced eosinophil accumulation. |
| Institute | Institute of Applied Molecular Medicine |
| Last Name | Zubeldia-Varela |
| First Name | Elisa |
| Address | Avda. Monteprincipe s/n 28668 Boadilla del Monte, Madrid, España |
| elisa.zubeldiavarela@ceu.es | |
| Phone | Tlf: 91 372 47 00 ext. 14675 |
| Submit Date | 2022-07-19 |
| Num Groups | 4 groups: BALB/c wild type (WT) and IgE knock-in mice (C.Ighg1tm1.1Pyu) in the BALB/c background, both sensitised and non-sensitised to ovalbumin. |
| Total Subjects | 29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC/LC-MS |
| Release Date | 2022-08-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003646 | AN003647 | AN003648 | AN003649 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | CE | GC |
| Chromatography system | Agilent 1290 | Agilent 1290 | Agilent 7100 CE | Agilent 7890A |
| Column | Zorbax Extend C18 (50 x 2.1 mm,1.8um) | Zorbax Extend C18 (50 x 2.1 mm,1.8um) | Fused-silica capillary (total length,100 cm;,50um) | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | ESI | ESI | ESI | EI |
| MS instrument type | QTOF | QTOF | TOF | Single quadrupole |
| MS instrument name | Agilent 6545 QTOF | Agilent 6545 QTOF | Agilent 6224 TOF | Agilent 5975C |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | POSITIVE |
| Units | Peak Area | peak area | Peak Area | Peak area |
MS:
| MS ID: | MS003397 |
| Analysis ID: | AN003646 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | LC-MS analysis was performed on an Agilent HPLC system (1290 infinity II series, Agilent Technologies, Waldbronn, Germany), equipped with a degasser, two binary pumps, and a thermostated autosampler coupled with quadrupole-time of flight analyzer (Q-TOF), LC-MS (6545) system (Agilent Technologies, Waldbronn, Germany) |
| Ion Mode: | POSITIVE |
| MS ID: | MS003398 |
| Analysis ID: | AN003647 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | LC-MS analysis was performed on an Agilent HPLC system (1290 infinity II series, Agilent Technologies, Waldbronn, Germany), equipped with a degasser, two binary pumps, and a thermostated autosampler coupled with quadrupole-time of flight analyzer (Q-TOF), LC-MS (6545) system (Agilent Technologies, Waldbronn, Germany) |
| Ion Mode: | NEGATIVE |
| MS ID: | MS003399 |
| Analysis ID: | AN003648 |
| Instrument Name: | Agilent 6224 TOF |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | CE-MS analysis was performed by a CE system (Agilent Technologies 7100) coupled to a time of flight analyzer (TOF; Agilent Technologies 6224) |
| Ion Mode: | POSITIVE |
| MS ID: | MS003400 |
| Analysis ID: | AN003649 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | GC-MS analysis was performed by a GC system (Agilent Technologies 7890A) equipped with an autosampler (Agilent 7693) coupled to a mass spectrometer with triple-Axis detector (5975C, Agilent). |
| Ion Mode: | POSITIVE |
