Summary of Study ST002380
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001530. The data can be accessed directly via it's Project DOI: 10.21228/M8XH73 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002380 |
| Study Title | Metabolomic and Cultivation Insights into the Tolerance of the Spacecraft-Associated Acinetobacter Towards Kleenol 30, a Cleanroom Floor Detergent |
| Study Type | research |
| Study Summary | To ensure cleanliness, NASA spacecraft are assembled in cleanroom facilities, where floors are routinely cleansed with Kleenol 30 (K30), an alkaline detergent. Through metabolomic and cultivation approaches, we show that cultures of spacecraft-associated Acinetobacter tolerate up to 1% v/v K30 and are fully inhibited at ≥2%; in comparison, NASA cleanrooms are cleansed with 0.8% K30. For A. johnsonii 2P08AA (isolated from a cleanroom floor), cultivations with 0.1% v/v K30 yield (1) limited changes in the intracellular metabolome and (2) increases in extracellular sugar acids, monosaccharides, organic acids, and fatty acids. For A. radioresistens 50v1 (isolated from a spacecraft surface), cultivations yield (1) differential changes in intracellular amino acids, compatible solutes, nucleotide-related metabolites, dicarboxylic acids, and saturated fatty acids and (2) substantial yet differential impacts to extracellular sugar acids, monosaccharides, and organic acids. These combined results suggest that (1) K30 manifests strain-dependent impacts on the intracellular metabolomes and (2) K30 influences extracellular trace element acquisition in both strains. Hence, this work lends support towards the hypothesis that repeated cleansing during spacecraft assembly serve as selective pressures that promote tolerances towards the cleaning conditions. |
| Institute | California State Polytechnic University, Pomona |
| Department | Chemistry & Biochemistry |
| Last Name | mogul |
| First Name | rakesh |
| Address | 3801 w. temple ave. |
| rmogul@cpp.edu | |
| Phone | 9098694309 |
| Submit Date | 2022-11-04 |
| Analysis Type Detail | GC-MS |
| Release Date | 2022-12-06 |
| Release Version | 1 |
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Combined analysis:
| Analysis ID | AN003879 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC x GC-TOF |
| MS instrument name | Leco Pegasus IV TOF |
| Ion Mode | POSITIVE |
| Units | counts |
MS:
| MS ID: | MS003620 |
| Analysis ID: | AN003879 |
| Instrument Name: | Leco Pegasus IV TOF |
| Instrument Type: | GC x GC-TOF |
| MS Type: | EI |
| MS Comments: | Electron ionization was conducted as 70 eV with an ion source temperature of 250°C. Spectra were recorded using an acquisition rate of 17 scans/second across a mass range of 85-500 Da. |
| Ion Mode: | POSITIVE |
