Summary of Study ST003297
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002048. The data can be accessed directly via it's Project DOI: 10.21228/M8WG0S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003297 |
| Study Title | Metabolomic profiling of cultured TRAMP-C2 cells in the presence or absence of PD-L1. |
| Study Summary | Recent evidence suggests that PD-L1, well-known as the ligand of the immune inhibitory receptor PD-1, can have cell-intrinsic effects in cancer and immune cells. One such cell-intrinsic effect is modulation of cellular metabolism, including regulation of mTOR activity and glycolysis. Here, we analyzed the metabolome of cultured mouse prostate cancer cells (TRAMP-C2) expressing PD-L1 or with PD-L1 deleted via CRISPR/Cas9. |
| Institute | University of Ottawa |
| Last Name | Hodgins |
| First Name | Jonathan |
| Address | 451 Smyth Rd, Ottawa, ON K1H 8M5, Canada |
| jonathanhodgins17@gmail.com | |
| Phone | 613-562-5800 |
| Submit Date | 2024-05-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-07-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005401 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity II |
| Column | Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6470A |
| Ion Mode | NEGATIVE |
| Units | uM |
MS:
| MS ID: | MS005128 |
| Analysis ID: | AN005401 |
| Instrument Name: | Agilent 6470A |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Multiple reaction monitoring (MRM) transitions were optimized using authentic standards and quality control samples. Metabolites were quantified by integrating the area under the curve of each compound using external standard calibration curves with Mass Hunter Quant (Agilent). No corrections for ion suppression or enhancement were applied. |
| Ion Mode: | NEGATIVE |
