Summary of Study ST003361
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002087. The data can be accessed directly via it's Project DOI: 10.21228/M8V825 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003361 |
| Study Title | Bile acid–induced gut mitochondrial dysfunction promotes Enterobacteriaceae colonization and associates with dysbiosis in Crohn's disease |
| Study Summary | Here, we investigated whether bile acids influence gut colonization by Enterobacteriaceae. In an external human dataset, we found association between mucosal Enterobacteriaceae and the expression of mitochondrial damage–related genes. In a murine model, increased intestinal BA availability enhanced colonization by E. coli in an oxygen-dependent manner. Overall, this study establishes BAs as a potential bridge that facilitates interactions between commensals and the host epithelium, laying the essential foundation for future investigations into various intestinal diseases. |
| Institute | University of British Columbia |
| Last Name | Finlay |
| First Name | Brett |
| Address | office: MSL 333 lab: MSL 363 |
| bfinlay@msl.ubc.ca | |
| Phone | 604-822-2210 |
| Submit Date | 2024-07-30 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-08-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005503 |
|---|---|
| Chromatography ID | CH004185 |
| MS ID | MS005229 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Bruker Impact II |
| Ion Mode | NEGATIVE |
| Units | Relative abundance |
MS:
| MS ID: | MS005229 |
| Analysis ID: | AN005503 |
| Instrument Name: | Bruker Impact II |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | To ensure strict quality control and evaluate the performance of the instrument, 20 µL aliquots were taken from each individual sample and combined to form a quality control sample (QC). This QC sample was then injected after every five samples, which helped in assessing the stability of the instrument and the coefficient of variation (CV) associated with the detected ions. Raw data processing was performed using Progenesis QI™ software (V3.0.7600.27622) incorporating METLIN™ plugin V1.0.7642.33805 (NonLinear Dynamics, Newcastle Upon Tyne, UK). This included peak picking, alignment, normalization and integration. Statistical analysis was performed using peak area. |
| Ion Mode: | NEGATIVE |
