Summary of Study ST003435
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001941. The data can be accessed directly via it's Project DOI: 10.21228/M8TM76 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003435 |
Study Title | Metabolomics analysis of zebrafish embryos treated with rotenone, Fasnall, TVB-2640, and GSK2194069 |
Study Summary | To compare the metabolic effects and toxicity of Fasnall with rotenone in vivo, zebrafish embryos 48 h post-fertilization were exposed to a drug-containing medium for 6 h. Rotenone at 25 nM is lethal to fish embryos, while 5 nM concentration leads to a ~15-fold lactate accumulation. Similarly, Fasnall treatment increases lactate content, although the magnitude of the effect is significantly lower. Unlike 5 nM rotenone, Fasnall treatment does not cause visible phenotypic changes in the yolk. The zebrafish model suggests that Fasnall acts as a Complex I inhibitor in vivo. |
Institute | Wistar Institute |
Department | Molecular and Cellular Oncogenesis Program, Ellen and Ronald Caplan Cancer Center |
Laboratory | Schug's Lab |
Last Name | Mukha |
First Name | Dzmitry |
Address | 3601 Spruce St, Philadelphia, PA 19104, USA |
dmukha@wistar.org | |
Phone | +12154956903 |
Submit Date | 2024-08-21 |
Num Groups | 16 |
Total Subjects | 48 |
Publications | Submission Pending |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML, wiff |
Analysis Type Detail | LC-MS |
Release Date | 2024-09-12 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005643 | AN005644 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Shimadzu 20AD | Shimadzu 20AD |
Column | Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um) | Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
Ion Mode | NEGATIVE | POSITIVE |
Units | Counts per second (cps) | Counts per second (cps) |
MS:
MS ID: | MS005367 |
Analysis ID: | AN005643 |
Instrument Name: | ABI Sciex 5500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Data were analyzed with SCIEX Multiquant 3.0.3. |
Ion Mode: | NEGATIVE |
Capillary Temperature: | 500 °C |
Capillary Voltage: | -4500 |
Dry Gas Flow: | 70 |
Dry Gas Temp: | 500 °C |
Ion Source Temperature: | 500 °C |
Ion Spray Voltage: | -4500 |
Mass Accuracy: | UNIT |
Source Temperature: | 500 °C |
Spray Voltage: | -4500 |
Desolvation Gas Flow: | 70 |
Desolvation Temperature: | 500 °C |
MS ID: | MS005368 |
Analysis ID: | AN005644 |
Instrument Name: | ABI Sciex 5500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Data were analyzed with SCIEX Multiquant 3.0.3. |
Ion Mode: | POSITIVE |
Capillary Temperature: | 500 °C |
Capillary Voltage: | 4500 |
Dry Gas Flow: | 70 |
Dry Gas Temp: | 500 °C |
Ion Source Temperature: | 500 °C |
Ion Spray Voltage: | 4500 |
Mass Accuracy: | UNIT |
Source Temperature: | 500 °C |
Spray Voltage: | 4500 |
Desolvation Gas Flow: | 70 |
Desolvation Temperature: | 500 °C |