Summary of Study ST003532
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002173. The data can be accessed directly via it's Project DOI: 10.21228/M8R23J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003532 |
| Study Title | Micropeptide hSPAR, a glutamine regulator, suppresses tumor growth via TRIM21-P27KIP1-mTOR pathway - HEK293T human embryonic kidney cells |
| Study Summary | The microprotein hSPAR can specifically regulate the mTOR signaling activity and cell proliferation in breast cancer cells. However, such regulatory effects of hSPAR are not applicable in HEK293T cells. Metabolic data of amino acids indicate that overexpressing hSPAR does not affect the content of amino acids in HEK293T cells, including glutamine. The metabolic variations in amino acids triggered by hSPAR in different cell types may be the underlying molecular mechanism for the distinct regulatory effects of hSPAR. |
| Institute | University Of Science And Technology of China |
| Last Name | Wang |
| First Name | Wei |
| Address | 443 Huangshan Road, Hefei city, Anhui Province, 230022, China |
| WW571@mail.ustc.edu.cn | |
| Phone | 18604523231 |
| Submit Date | 2024-10-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-01-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005802 | AN005803 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | SCIEX ExionLC AD | SCIEX ExionLC AD |
| Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | ng/mg of cells | ng/mg of cells |
MS:
| MS ID: | MS005522 |
| Analysis ID: | AN005802 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | USTC_Protocol.pdf |
| MS ID: | MS005523 |
| Analysis ID: | AN005803 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | USTC_Protocol.pdf |
