Summary of Study ST003623
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002208. The data can be accessed directly via it's Project DOI: 10.21228/M86Z6P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003623 |
| Study Title | NRF2 supports non-small cell lung cancer growth independently of CBP/p300-enhanced glutathione synthesis: Global metabolomics analysis on A549 cells at different NRF2 status (Part 1 of 3) |
| Study Type | Untargeted Metabolomics |
| Study Summary | This study aims at discovering metabolic changes in A549 cancer cells in the presence and absence of NRF2, and a mutant NRF2 genotypes. Metabolites were extracted from cell pellets by using an LLE method with Methanol/Chloroform/water. The aqueous layer was analyzed by HILIC-HRMS. An in-house RT library was used to identify metabolites. Statistical analyses was performed to identify statistically significant changes in the metabolism. 35 metabolites presented differential abundance between NRF2 knockdown and wildtype conditions. In particular, GSH and several glutamate dipeptides were significantly depleted upon NRF2 knockdown, in line with the prevailing role of NRF2 in controlling GSH biosynthesis. Disruption of additional metabolites involved in the PPP (sedoheptulose-7-phosphate, S7P), nucleotide (CMP, IMP) and amino acid (kynurenine, homocitrulline) metabolism were also observed upon NRF2 knockdown. |
| Institute | Genentech Inc. |
| Last Name | Wang |
| First Name | Mike |
| Address | 1 DNA Way, South San Francisco, CA 94080, USA |
| wang.mike@gene.com | |
| Phone | 6502457991 |
| Submit Date | 2024-12-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-01-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005953 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Shimadzu Nexera HPLC |
| Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm × 1.7μm, 130Å) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | Ion Count |
MS:
| MS ID: | MS005668 |
| Analysis ID: | AN005953 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Samples were run in polarity switching mode for both positive and negative acquisitions. The Q Exactive Plus Mass Spectrometer was operated with the following parameters: Sheath gas flow rate, 50 units; Aux gas flow rate, 13 units; Aux gas temperature, 425 °C; Capillary temperature, 263°C; Spray voltage, 3500V for pos and -2500V for neg; Scan mode, Full MS scan with data-dependent MS/MS acquisition. In Full MS scan, scan range is 60-900 m/z; resolution is 70,000; AGC target, 1×e^6; Maximum IT, 200 ms. In ddMS2 scan, top 5 ions are selected with an isolation window of 1.5 m/z; resolution is 17,500; AGC target, 5×e^4; Maximum IT, 20 ms. |
| Ion Mode: | UNSPECIFIED |
