Summary of Study ST003643
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002253. The data can be accessed directly via it's Project DOI: 10.21228/M8DC25 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003643 |
| Study Title | Metabolite analysis for WT and BnaMYB52 mutants by LC-MS/MS |
| Study Summary | BnaA09.MYB52 directly targets the BnaBAN promoters and promotes BnaBAN expression in Brassica napus. BAN, encoding anthocyanidin reductase that converts anthocyanidins to 2,3- cis-flavan-3-ols compounds (proanthocyanidins starter units), is involved in the flavonoid biosynthesis pathway. Thus, Metabolite analysis was conducted to detect the content of flavonoid in WT (Wild-type), OE (BnaA09.MYB52 overexpression lines in the genetic background Westar) and mutants (four homologous genes of BnaMYB52 knocked out) plants. About 0.1 g mature seeds were collected from WT, OE and mutant plants. Metabolites analysis demonstrated that BnaMYB52 positively regulated the content of several metabolites (such as L-phenylalanine, p-coumaric acid, grosvenorine and astragalin) in flavonoid pathway. |
| Institute | Huazhong Agricultural University |
| Last Name | Jiang |
| First Name | Ye |
| Address | Shi Zishan Street 1th, Wuhan, Hubei, 430070, China |
| vyejiang@163.com | |
| Phone | 13697353446 |
| Submit Date | 2024-12-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-01-26 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005982 |
|---|---|
| Chromatography ID | CH004544 |
| MS ID | MS005695 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu 20AD |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 6500 QTrap |
| Ion Mode | POSITIVE |
| Units | Peak area |
MS:
| MS ID: | MS005695 |
| Analysis ID: | AN005982 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Acquisition parameters: A scheduled multiple reaction monitoring method was aplied based on comercial standards, with an MRM detection window of 90 s and a target scan time of 1.5 s. For those appeared within the MRM window and have similar MS2 fragments with designated standards, these metabolites that have different retention time are named as isomers. The acquisition volumn is 2 ul. Peak identification criteria: Compared with commercial standards，with retention time of ±0.2 min. Software used: Analyst 1.7.1 with default criteria. |
| Ion Mode: | POSITIVE |
