Summary of Study ST003808
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002382. The data can be accessed directly via it's Project DOI: 10.21228/M8QZ7Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003808 |
| Study Title | Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division |
| Study Summary | Peroxisomes play a crucial role in cellular metabolism. Glucose-6-phosphate dehydrogenase (G6PD), the gatekeeper enzyme of the pentose phosphate pathway, is primarily localized in the cytosol. However, studies have reported its presence in peroxisomes as well. This project aims to determine the function of G6PD on the peroxisomal membrane. In this study, we overexpressed G6PD in either the cytosol or on the peroxisomal membrane of mammary epithelial stem-like cells (hMECs). By comparing their lipidomic profiles, we found that peroxisomal membrane-associated G6PD provides NADPH, which feeds into peroxisomal ether lipid synthesis. |
| Institute | University of Helsinki |
| Department | Faculty of Biological and Environmental Sciences |
| Laboratory | Katajisto Laboratory |
| Last Name | Hien |
| First Name | Bui |
| Address | Biocenter 1, Viikinkaari 9 |
| hien.bui@helsinki.fi | |
| Phone | +358294159407 |
| Submit Date | 2025-03-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf, mzdata.xml |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2025-03-24 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN006261 | AN006262 |
|---|---|---|
| Chromatography ID | CH004749 | CH004750 |
| MS ID | MS005963 | MS005964 |
| Analysis type | MS | MS |
| Chromatography type | GC | Reversed phase |
| Chromatography system | Shimadzu GC-2010 | Agilent 1290 Infinity |
| Column | Phenomenex Zebron ZB-wax capillary (30m x 0.25mm, 0.25um) | Phenomenex Luna Omega C18 (50 x 2.1mm, 1.6um, 100 Å) |
| MS Type | EI | ESI |
| MS instrument type | GC-FID & GC-MSD | Triple quadrupole |
| MS instrument name | Shimadzu QP2010 Plus | Agilent 6490 QQQ |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | pmol/sample | peak area |
MS:
| MS ID: | MS005963 |
| Analysis ID: | AN006261 |
| Instrument Name: | Shimadzu QP2010 Plus |
| Instrument Type: | GC-FID & GC-MSD |
| MS Type: | EI |
| MS Comments: | The samples were injected into a GC-2010 Plus gas chromatograph (Shimadzu Scientific Instruments, Kyoto, Japan) with flame-ionization detector (FID) for quantification of the analytes. Both GC systems were equipped with Zebron ZB-wax capillary columns (30 m, 0.25 mm ID and film thickness 0.25 μm; Phenomenex, Torrence CA, USA). GC-FID chromatographic peak areas were converted to mol% using the theoretical correction factors for FID, and the FOHs were calculated as µmol/1 million cells (ebook ISBN:9780429127557). The identifications were performed using a GCMS-QP2010 Ultra (Shimadzu Scientific Instruments, Kyoto, Japan) with mass selective detector (MSD). The spectra of the analytes were compared with the spectra of several authentic standard mixtures of FAMEs (Supelco CRM47885, 47033, 47085-U, 47015-U and 47080-U) and published reference mass spectra (https://lipidmaps.org/resources/lipid_web?page=ms/masspec.html). |
| Ion Mode: | POSITIVE |
| MS ID: | MS005964 |
| Analysis ID: | AN006262 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | PE plasmalogens (PE P) were identified with alkenyl chain (16:0, 18:0, 18:1)-specific scans and quantified from MS- scan. Mass spectra were processed using MassHunter Qualitative Navigator software (Agilent) and lipid species were quantified utilizing the internal and additional external standards (glucosylceramide 18:1;O2/24:1) and LIMSA software. Lipid data are expressed as molar percentages (mol%) and lipid species are marked as follows: [sum of acyl chain carbons]: [sum of acyl chain double bonds] (e.g., PC 36:1). |
| Ion Mode: | NEGATIVE |
