Summary of Study ST000019
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000018. The data can be accessed directly via it's Project DOI: 10.21228/M8Z59Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000019 |
| Study Title | Determine purity and quality of IROA labelled glucose |
| Study Type | NMR and MS analysis |
| Study Summary | We demonstrate the global metabolic analysis ofCaenorhabditis elegansstress responses using a mass-spectrometry-based technique called isotopic ratio outlier analysis (IROA). In an IROA protocol, control and experimental samples are isotopically labeled with 95 and 5%13C, and the two sample populations are mixed together for uniform extraction, sample preparation, and LC-MS analysis.To illustrate the utility of IROA for global metabolomics, we exposed wild-type (N2) worms to a heat shock (30 min heat shock at 33 C), which causes significant, widespread changes in metabolism. We collected and analyzed material from the exometabolome (all material that worms release in the supernatant) and the endometabolome (homogenized total extracts from the worm bodies). |
| Institute | University of Florida |
| Department | Biochemistry & Molecular Biology |
| Last Name | Stupp |
| First Name | Gregory |
| stuppie@ufl.edu | |
| Submit Date | 2013-12-10 |
| Study Comments | Determine purity and quality of IROA labelled glucose |
| Raw Data Available | Yes |
| Raw Data File Type(s) | ser |
| Uploaded File Size | 90 M |
| Analysis Type Detail | NMR |
| Release Date | 2014-03-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
| NMR ID: | NM000004 |
| Analysis ID: | AN000037 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Standard Concentration: | 10 mM |
| Spectrometer Frequency: | 500 MHz |
| NMR Probe: | 5mm FBBO-Z Probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm |
| Pulse Sequence: | zg |
| Pulse Width: | 4 |
| Receiver Gain: | 152 |
| Chemical Shift Ref Cpd: | TSP |
| Temperature: | 27 |
| Number Of Scans: | 16 |
| Acquisition Time: | 4 s |
| Relaxation Delay: | 3 s |
| Spectral Width: | 5498.5 |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 16384 |
| Line Broadening: | 0.33 Hz |
| Zero Filling: | Yes |
| Apodization: | Exponential |
| Baseline Correction Method: | Whittaker Smoother |
| Chemical Shift Ref Std: | TSP |
