Summary of study ST000102

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000094. The data can be accessed directly via it's Project DOI: 10.21228/M8X309 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000102
Study TitleCold adaptation shapes the robustness of metabolic networks in Drosophila melanogaster
Study TypeTime course during cold exposure for four genetic lines of flies
Study SummaryMetabolites of cold hardy versus cold susceptible flies were compared using N less than R-based metabolomics. We used 8 replicates per line (2 hardy lines, two susceptible lines), and sampled each line at three time points (before, during and after cold), giving rise to 96 samples total.
Institute
University of Florida
DepartmentEntomology and Nematology
Last NameWilliams
First NameCaroline
Emailcmw@berkeley.edu
Submit Date2014-09-26
Num Groups4
Total Subjects96
Raw Data AvailableYes
Raw Data File Type(s).txt,out, .xml, .par, pdata directory has binary files
Uploaded File Size151 M
Analysis Type DetailNMR
Release Date2015-02-03
Release Version1
Caroline Williams Caroline Williams
https://dx.doi.org/10.21228/M8X309
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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NMR:

NMR ID:NM000036
Analysis ID:AN000169
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Field Frequency Lock:-6958.1
Standard Concentration:1 mM TMSP
Spectrometer Frequency:700 MHz
NMR Probe:Room Temperature 5-mm triple resonance probe
NMR Solvent:D2O
NMR Tube Size:5 mm
Shimming Method:topshim (automatic shimming)
Pulse Sequence:noesypr1d
Water Suppression:presaturation
Pulse Width:9.4-9.6 ?s
Power Level:-1.00 dB
Receiver Gain:200
Offset Frequency:3294.98 Hz
Presaturation Power Level:53.64 dB
Chemical Shift Ref Cpd:TMSP
Temperature:298 K
Number Of Scans:120
Dummy Scans:4
Acquisition Time:2.9 s
Relaxation Delay:4 s
Spectral Width:16.0872 ppm
Num Data Points Acquired:65536
Real Data Points:32768
Line Broadening:0.3 Hz
Zero Filling:2x
Apodization:exponential multiply (em)
Baseline Correction Method:abs (automatic baseline correction with integration (1D))
Chemical Shift Ref Std:0.00 ppm
  
NMR ID:NM000037
Analysis ID:AN000170
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:2D 1H-13C HSQC
Field Frequency Lock:-6958.1
Standard Concentration:1 mM TMSP
Spectrometer Frequency:700 MHz
NMR Probe:Room Temperature 5-mm triple resonance probe
NMR Solvent:D2O
NMR Tube Size:5 mm
Shimming Method:topshim (automatic shimming)
Pulse Sequence:HSQCEDETGPSISP2.2
Pulse Width:9.4-9.6 ?s
Power Level:f1 -1.00 dB, f2 -4.13 dB
Receiver Gain:2050
Offset Frequency:f2 3294.95 Hz, f1 14081.41 Hz
Chemical Shift Ref Cpd:TMSP
Temperature:298 K
Number Of Scans:256
Dummy Scans:256
Acquisition Time:f2 0.13317 s, f1 0.0083333 s
Relaxation Delay:1.5 s
Spectral Width:f2 10.9888, f1 180.00
Num Data Points Acquired:f2 2048, f1 528
Real Data Points:f2 4096, f1 2048
Line Broadening:f2 1.0 Hz, f1 0.3 Hz
Zero Filling:f2 1x, f1 4x
Apodization:f2 QSINE, f1 QSINE
Chemical Shift Ref Std:0.00, 0.00 ppm
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