Summary of Study ST000150

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000131. The data can be accessed directly via it's Project DOI: 10.21228/M8WW2P This work is supported by NIH grant, U2C- DK119886.

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Study IDST000150
Study TitleAssociation of Metabolic Profile and Microbiome in Chronic Pressure Ulcer Wounds
Study SummaryChronic, non-healing wounds contribute significantly to the suffering of patients with co-morbidities in the clinical population with mild to severely compromised immune systems. Normal wound healing proceeds through a well-described process. However, in chronic wounds this process seems to become dysregulated at the transition between resolution of inflammation and re-epithelialization. Bioburden in the form of colonizing bacteria is a major contributor to the delayed headlining in chronic wounds such as pressure ulcers. However how the microbiome influences the wound metabolic landscape is unknown. Here, we have used a Systems Biology approach to determine the association between the taxonomic and metabolomic profile of wounds colonized by bacteria. Pressure ulcer biopsies were harvested from primary chronic wounds and bisected into top and bottom sections prior to analysis of microbiome by pyrosequencing and analysis of metabolome using 1H nuclear magnetic resonance (NMR) spectroscopy. Bacterial taxonomy revealed that wounds were colonized predominantly by three main phyla, but differed significantly at the genus level. While taxonomic profiles demonstrated significant variability between wounds, metabolic profiles shared significant similarity based on the depth of the wound biopsy. Association between taxonomy and metabolic landscape indicated significant wound-to-wound similarity in metabolite enrichment sets and metabolic pathway impacts, especially with regard to amino acid metabolism. To our knowledge, this is the first demonstration of a statistically robust correlation between bacterial colonization and metabolic landscape within the chronic wound environment.
Institute
Montana State University
DepartmentChemistry and Biochemistry
LaboratoryAmmons
Last NameAmmons
First NameMary Cloud
Address103 CBB, Montana State University, Bozeman, MT 59717
Emailmcammons@chemistry.montana.edu
Phone406-600-0301
Submit Date2015-03-10
Num GroupsNA
Total Subjects4 patients/8 samples
Raw Data AvailableNo
Analysis Type DetailNMR
Release Date2015-04-20
Release Version1
Mary Cloud Ammons Mary Cloud Ammons
https://dx.doi.org/10.21228/M8WW2P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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NMR:

NMR ID:NM000052
Analysis ID:AN000237
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:600 MHz
Offset Frequency:600 mHz
Presaturation Power Level:cryoprobeTM
Chemical Shift Ref Cpd:10mM NaH2PO4/ Na2HPO4
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