Summary of Study ST000607

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000444. The data can be accessed directly via it's Project DOI: 10.21228/M8NW2H This work is supported by NIH grant, U2C- DK119886.

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Study IDST000607
Study Title1D-1H-Nuclear Magnetic Resonance Metabolomics Reveals Age-related Changes in Metabolites Associated with Experimental Venous Thrombosis
Study TypeExperimental venous thrombosis
Study SummarySodium heparin preserved whole blood samples were collected from young and old mice with and without induced VT. Samples were subjected to MeOH:CHCl3 extraction and the MeOH was assayed by 1H-NMR. Chenomx software was used for spectral analysis.
Institute
University of Michigan
DepartmentClinical Pharmacy
LaboratoryThe NMR Metabolomics Laboratory (Stringer)
Last NameStringer
First NameKathleen
AddressCollege of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109
EmailNMRmetabolomics@umich.edu
PhoneNA
Submit Date2016-12-08
Num Groups4
Total Subjects31
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2017-07-10
Release Version1
Kathleen Stringer Kathleen Stringer
https://dx.doi.org/10.21228/M8NW2H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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NMR:

NMR ID:NM000104
Analysis ID:AN000928
Instrument Name:Agilent 500/54 Premium Shielded VNMRS system
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Standard Concentration:10% (~0.5mM)
Spectrometer Frequency:500 MHz
NMR Probe:ONE-Probe
NMR Solvent:D2O
NMR Tube Size:5mm
Shimming Method:Auto shim (gradient shimming)
Pulse Sequence:1D-NOESY
Water Suppression:saturation at 80 Hz induced field strength
Pulse Width:5.5ms
Offset Frequency:around -178Hz
Chemical Shift Ref Cpd:6mM Formate
Temperature:25°C
Number Of Scans:32
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