Summary of Study ST000242

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000195. The data can be accessed directly via it's Project DOI: 10.21228/M8630S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000242
Study TitleWhole unconditioned medium (Defined culture media, M199),Whole M1 medium,Whole M2 medium
Study Typedifferential metabolomics
Study Summary Media was flash frozen with N2 and stored at -80 C. Samples can be stored at -80 C until use. ~1 ml aliquots. The following media has been provided to the metabolomics core in biological triplicates. Complete (Whole) Media: 1) Whole unconditioned medium (Defined culture media, M199) 2) Whole M1 medium 3) Whole M2 medium
Institute
Mayo Clinic
DepartmentPhysiology and Biomedical Engineering
Last NameFarrugia
First NameGianrico
EmailDasari.Surendra@mayo.edu
Submit Date2015-06-26
Num Groups1
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-07-08
Release Version2
Gianrico Farrugia Gianrico Farrugia
https://dx.doi.org/10.21228/M8630S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000195
Project DOI:doi: 10.21228/M8630S
Project Title:Metabolimic Analysis of Conditioned Macrophage Media
Project Type:Untargeted LC-MS Metabolomics
Project Summary:We are studying the secreted components from pro-inflammatory macrophages, coined M1. We use a macrophage at the opposite end of the macrophage phenotype spectrum as a control, the anti-inflammatory macrophage, coined M2. M1 macrophage conditioned media has a <3 kD factor that reduces survival of a cell of interest in primary culture. This factor is not present in M2 macrophage conditioned media <3 kD, as there is no effect on primary cell numbers. The goal of this project is to analyze the components present in the M1 conditioned <3 kD media and compare to the M2 conditioned < 3kD media.
Institute:Mayo Clinic
Department:Physiology and Biomedical Engineering
Last Name:Farrugia
First Name:Gianrico
Address:200 First Street SW, Rochester, MN 55905
Email:Dasari.Surendra@mayo.edu
Phone:507-284-0513
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