Summary of Study ST000274

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000218. The data can be accessed directly via it's Project DOI: 10.21228/M86W2G This work is supported by NIH grant, U2C- DK119886.

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Study IDST000274
Study TitleHIF 1 alpha type 2 cells metabolomics
Study TypeGlycolysis/TCA/Nucleotide analysis (tissue/cells)
Study SummaryLung contusion is a major risk factor for the development of acute respiratory distress syndrome. Hypoxia-inducible factor-1? is the primary transcription factor that is responsible for regulating the cellular response to changes in oxygen tension. We set to determine if hypoxia-inducible factor-1? plays a role in the pathogenesis of acute inflammatory response and injury in lung contusion. Nonlethal closed-chest unilateral lung contusion was induced in a hypoxia reporter mouse model and type 2 cell-specific hypoxia-inducible factor-1? conditional knockout mice. The mice were killed at 5-, 24-, 48-, and 72-hour time points, and the extent of systemic and tissue hypoxia was assessed. In addition, injury and inflammation were assessed by measuring bronchoalveolar lavage cells (flow cytometry and cytospin), albumin (permeability injury), and cytokines (inflammation). Isolated type 2 cells from the hypoxia-inducible factor-1? conditional knockout mice were isolated and evaluated for proinflammatory cytokines following lung contusion. Finally, the role of nuclear factor-?B and interleukin-1? as intermediates in this interaction was studied. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245055/
Institute
University of Michigan
DepartmentSurgery
LaboratoryRaghavendran Lab
Last NameThomas
First NameBivin
AddressAnn Arbor, MI
Emailbthomas@umich.edu
Phone734-615-7142
Submit Date2014-06-04
Num Groups4
Total Subjects8
Study Commentshttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245055/ : HIF1 (+/+) stands for hypoxia reporter mouse model
HIF1 (-/-) stands for type 2 cell-specific hypoxia-inducible factor-1? conditional knockout
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-01-13
Release Version1
Bivin Thomas Bivin Thomas
https://dx.doi.org/10.21228/M86W2G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000218
Project DOI:doi: 10.21228/M86W2G
Project Title:HIF 1 alpha
Project Summary:Glycolysis/TCA/Nucleotide analysis (AEC cells)
Institute:University of Michigan
Department:Surgery
Laboratory:Raghavendran Lab
Last Name:Raghavendran
First Name:Krishnan
Address:Ann Arbor, MI
Email:kraghave@umich.edu
Phone:734-615-7142
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