Summary of Study ST000456

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000352. The data can be accessed directly via it's Project DOI: 10.21228/M8JK5M This work is supported by NIH grant, U2C- DK119886.

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Study IDST000456
Study TitleMetabolomic changes during active immunization with anti-METH vaccines.
Study SummaryA targeted metabolomics analysis was performed on serum samples from rats immunized with a methamphetamine-like hapten covalently bound to keyhole limpet hemocyanin (KLH) monomers, and controls. The subjects were approximately 300 g adult male Sprague Dawley rats. RTI International RCMRC received pre-immune serum from RI 11-03A (n=12) which is a matched control serum that was collected before starting immunizations, and week 17 bleeds from RI 11-03A (n=12), RI 11-03B (n=8), and RI 11-03C (n=12). The A-C nomenclature denotes A) complete antigen with adjuvant, B) complete antigen without adjuvant and C) only KLH carrier protein and adjuvant without a METH-like hapten. The rats’ serum samples were extracted and prepared for a combined flow injection (FIA) and LC-MS/MS assay (Biocrates AbsoluteIDQ® p180 kit). The Biocrates AbsoluteIDQ® p180 kit (Biocrates Life Sciences AG, Innsbruck, Austria) can quantitatively measure ~188 biologically relevant metabolites from five analyte groups (acylcarnitines, amino acids, biogenic amines, glycerophospho- and sphingolipids, and hexoses) on a triple quadrupole mass spectrometer.
Institute
University of North Carolina
DepartmentDiscovery-Sciences-Technology (DST)
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2016-08-31
Num Groups4
Total Subjects44
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-10-03
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8JK5M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000352
Project DOI:doi: 10.21228/M8JK5M
Project Title:Metabolomic changes during active immunization with anti-METH vaccines.
Project Summary:Metabolomics studies will be conducted on serum samples from rats immunized with a methamphetamine conjugate vaccine. The Subjects were approximately 300 g adult male Sprague Dawley rats. The following samples were sent to RTI International RCMRC: Pre-immune serum from RI 11-03A (n=12) which is a matched control serum that was collected before starting immunizations, and week 17 bleeds from RI 11-03A (n=12), RI 11-03B (n=8), and RI 11-03C (n=12). The A-C nomenclature denotes A) complete antigen with adjuvant, B) complete antigen without adjuvant and C) only KLH carrier protein and adjuvant without a METH-like hapten.
Institute:University of Arkansas
Department:Department of Pharmacology and Toxicology
Last Name:Owens
First Name:S. Michael
Address:4301 W. Markham St., #611 Little Rock, AR 72205 USA
Email:mowens@uams.edu
Phone:501-686-5487
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