Summary of Study ST000465
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000357. The data can be accessed directly via it's Project DOI: 10.21228/M8WW32 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000465 |
| Study Title | Uniquely Tumor-Selective Englerin A Profoundly Alters Lipid Metabolism in Renal Cell Carcinoma inducing ER-Stress and an Acute Inflammatory Response |
| Study Type | Metabolomic effect of Englerin A on renal cell carcinoma |
| Study Summary | This targeted metabolomic analysis was performed on renal cell carcinoma A498 cells with or without anti-cancer drug Englerin treatment for 24 and 48 h. |
| Institute | University of California, San Diego |
| Department | Department of Pediatrics |
| Last Name | Batova |
| First Name | Ayse |
| Address | La Jolla, CA 92093 |
| abatova@ucsd.edu | |
| Phone | 619-543-1962 |
| Submit Date | 2016-09-09 |
| Num Groups | Two groups for 24 h treatment (control and Eglerin treatment) and two groups for 48 h treatment. Each has 4 replicates |
| Total Subjects | 16 |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
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Project:
| Project ID: | PR000357 |
| Project DOI: | doi: 10.21228/M8WW32 |
| Project Title: | Uniquely Tumor-Selective Englerin A Profoundly Alters Lipid Metabolism in Renal Cell Carcinoma inducing ER-Stress and an Acute Inflammatory Response |
| Project Type: | Cancer cell |
| Project Summary: | Renal cell carcinoma (RCC) is among the top ten most common forms of cancer and is the most common malignancy of the kidney. Screening of plant extracts in search of new anti-cancer agents resulted in the discovery of englerin A, a guaiane sesquiterpene with potent cytotoxicity against renal cancer cells and a small subset of other cancer cells. the current study used a systems biology approach to explore the mechanism(s) of action of engerin A at a more global level.Our metabolomics analyses indicated that englerin A profoundly altered lipid metabolism in cc-RCC cell lines and generated significant levels of ceramides that were highly toxic to these cells. Microarray analyses determined that englerin A induced ER stress signaling and an acute inflammatory response, which was confirmed by quantitative PCR and Western Blot analyses.Our findings suggest that cc-RCC is highly sensitive to disruptions in lipid metabolism and ER stress and that these vulnerabilities can be targeted for the treatment of cc-RCC and possibly other lipid storing cancers. |
| Institute: | University of California, San Diego |
| Department: | Department of Pediatrics |
| Last Name: | Batova |
| First Name: | Ayse |
| Address: | La Jolla, CA 92093 |
| Email: | abatova@ucsd.edu |
| Phone: | 619-543-1962 |
