Summary of Study ST000469
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000360. The data can be accessed directly via it's Project DOI: 10.21228/M8HK59 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000469 |
| Study Title | Enterococcus faecalis nucleotide profiles - pilot |
| Study Type | Comparison between wild-type and mutant strain, as well as providing varying cell count input for WT |
| Study Summary | E. faecalis OG1RF and rel/relQ will be grown in FMC-AUG to OD600 = 0.25. 3E10 cells will be harvested by fast filtration, scraped off of membrane into ice-cold PBS, and pelleted by centrifugation. This process will be repeated with OG1RF only to harvest 3E9 and 3E8 cells. A second OG1RF sample of 3E10 cells will be scraped into formic acid; only acid-soluble extract willl be sent for this sample. |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | Lemos-Abranches |
| Last Name | Kajfasz |
| First Name | Jessica |
| Address | 1395 Center Drive, PO Box 100424 Gainesville, FL 32610 |
| jkajfasz@dental.ufl.edu | |
| Phone | 352-273-6675 |
| Submit Date | 2016-09-10 |
| Num Groups | 5 |
| Total Subjects | 5 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-10-03 |
| Release Version | 1 |
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Project:
| Project ID: | PR000360 |
| Project DOI: | doi: 10.21228/M8HK59 |
| Project Title: | Enterococcus faecalis nucleotie profiles |
| Project Summary: | Determination of metabolite profile of E. faecalis OG1RF control as compared to exposure to mupirocin or decoyinine |
| Institute: | University of Florida |
| Department: | Oral Biology |
| Laboratory: | Oral Biology Lemos-Abranches |
| Last Name: | Lemos |
| First Name: | Jose |
| Address: | 1395 Center Drive, PO Box 100424 Gainesville, FL 32610 |
| Email: | jlemos@dental.ufl.edu |
| Phone: | 352-273-8843 |
| Funding Source: | UF start-up funds |
