Summary of Study ST000570
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000418. The data can be accessed directly via it's Project DOI: 10.21228/M81880 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000570 |
| Study Title | Metabolome analysis of the cecal contents of GF mice and GF mice colonized with dominant gut microbes present in the ceca of neonatal and adult mice |
| Study Summary | Metabolome profiles of GF or GF mice reconstituted with Esherichia coli (EC), Bacteroides acidifaciens (Bac), or Clostridia consortium (CL) were compared. |
| Institute | Keio University |
| Department | Institute for Advanced Biosciences |
| Last Name | Fukuda |
| First Name | Shinji |
| Address | Tsuruoka, Yamagata 997-0052, Japan |
| sfukuda@sfc.keio.ac.jp | |
| Phone | +81-235-29-0528 |
| Submit Date | 2017-03-09 |
| Num Groups | 4 |
| Total Subjects | 17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-04-10 |
| Release Version | 1 |
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Project:
| Project ID: | PR000418 |
| Project DOI: | doi: 10.21228/M81880 |
| Project Title: | Metabolome analysis of the cecal contents of GF mice and GF mice colonized with dominant gut microbes present in the ceca of neonatal and adult mice |
| Project Summary: | The high susceptibility of neonates to infections has been assumed to be due to immaturity of the immune system, but the mechanism remains unclear. By colonizing adult germ-free mice with the cecal contents of neonatal and adult mice, we show that the neonatal microbiota is impaired in mediating colonization resistance against two major pathogens causing mortality in neonates. The lack of colonization resistance was caused by the absence of Clostridiales in the neonatal microbiota. Administration of Clostridiales, but not Bacteroidales, restored colonization resistance and abrogated intestinal pathology upon pathogen challenge. Conversely, depletion of Clostridiales abolished colonization resistance in adult mice. Furthermore, intragastric administration of Clostridiales protected neonatal mice from pathogen infection. The neonatal bacteria enhanced the ability of these protective Clostridiales to colonize the gut. These results identify the gut microbiota as a critical determinant of increased susceptibility to enteric infection during the neonatal period. |
| Institute: | Keio University |
| Department: | Institute for Advanced Biosciences |
| Last Name: | Fukuda |
| First Name: | Shinji |
| Address: | Tsuruoka, Yamagata 997-0052, Japan |
| Email: | sfukuda@sfc.keio.ac.jp |
| Phone: | +81-235-29-0528 |
