Summary of Study ST000570

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000418. The data can be accessed directly via it's Project DOI: 10.21228/M81880 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000570
Study TitleMetabolome analysis of the cecal contents of GF mice and GF mice colonized with dominant gut microbes present in the ceca of neonatal and adult mice
Study SummaryMetabolome profiles of GF or GF mice reconstituted with Esherichia coli (EC), Bacteroides acidifaciens (Bac), or Clostridia consortium (CL) were compared.
Keio University
DepartmentInstitute for Advanced Biosciences
Last NameFukuda
First NameShinji
AddressTsuruoka, Yamagata 997-0052, Japan
Submit Date2017-03-09
Num Groups4
Total Subjects17
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2018-04-10
Release Version1
Shinji Fukuda Shinji Fukuda application/zip

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Project ID:PR000418
Project DOI:doi: 10.21228/M81880
Project Title:Metabolome analysis of the cecal contents of GF mice and GF mice colonized with dominant gut microbes present in the ceca of neonatal and adult mice
Project Summary:The high susceptibility of neonates to infections has been assumed to be due to immaturity of the immune system, but the mechanism remains unclear. By colonizing adult germ-free mice with the cecal contents of neonatal and adult mice, we show that the neonatal microbiota is impaired in mediating colonization resistance against two major pathogens causing mortality in neonates. The lack of colonization resistance was caused by the absence of Clostridiales in the neonatal microbiota. Administration of Clostridiales, but not Bacteroidales, restored colonization resistance and abrogated intestinal pathology upon pathogen challenge. Conversely, depletion of Clostridiales abolished colonization resistance in adult mice. Furthermore, intragastric administration of Clostridiales protected neonatal mice from pathogen infection. The neonatal bacteria enhanced the ability of these protective Clostridiales to colonize the gut. These results identify the gut microbiota as a critical determinant of increased susceptibility to enteric infection during the neonatal period.
Institute:Keio University
Department:Institute for Advanced Biosciences
Last Name:Fukuda
First Name:Shinji
Address:Tsuruoka, Yamagata 997-0052, Japan